Okkema A Z, Visser S A, Cooper S L
Material Science Program, University of Wisconsin, Madison 53706.
J Biomed Mater Res. 1991 Nov;25(11):1371-95. doi: 10.1002/jbm.820251106.
Polyurethanes chain extended with N,N-bis (2-hydroxyethyl)-2-aminoethane-sulfonic acid (BES) were synthesized. The effect of the sulfonic acid group on the polymers' bulk, surface, and blood-contacting properties was evaluated by comparing the BES-based polymers with polyurethanes based on N-ethyldiethanolamine (EDEA). In addition, the effect of soft-segment polarity was addressed by comparing polyurethanes based on polytetramethylene oxide (PTMO) (MW = 1000) with polymers based on polyethylene oxide (PEO) (MW = 1000). The EDEA control samples had physical properties similar to a viscous fluid. The presence of the sulfonic acid group dramatically enhanced the degree of microphase separation and the mechanical strength of all the polymers. The more polar PEO soft segment resulted in polymers which were more phase mixed than the PTMO-based polyurethanes. Surface characterization studies revealed that in vacuum, all the surfaces were enriched in the polyether soft-segment phase. After 24-h equilibration in water, all the surfaces had similar surface polarities independent of the SO3H content. The canine ex vivo blood-contacting results showed that the sulfonic acid group in the PTMO-based polymers significantly reduced the number and activation of the adherent platelets. Fibrinogen deposition, however, increased with increasing sulfonic acid content. In contrast, platelet and fibrinogen deposition on the sulfonic acid-containing PEO-based polymers was greatly enhanced.
合成了用N,N-双(2-羟乙基)-2-氨基乙烷磺酸(BES)扩链的聚氨酯。通过将基于BES的聚合物与基于N-乙基二乙醇胺(EDEA)的聚氨酯进行比较，评估了磺酸基团对聚合物本体、表面及血液接触性能的影响。此外，通过比较基于聚四亚甲基醚二醇(PTMO)(分子量 = 1000)的聚氨酯与基于聚环氧乙烷(PEO)(分子量 = 1000)的聚合物，探讨了软段极性的影响。EDEA对照样品具有类似于粘性流体的物理性质。磺酸基团的存在显著提高了所有聚合物的微相分离程度和机械强度。极性更强的PEO软段导致聚合物比基于PTMO的聚氨酯具有更多的相混合。表面表征研究表明，在真空中，所有表面都富含聚醚软段相。在水中平衡24小时后，所有表面都具有相似的表面极性，与SO3H含量无关。犬离体血液接触结果表明，基于PTMO的聚合物中的磺酸基团显著减少了粘附血小板的数量和活化。然而，纤维蛋白原沉积随着磺酸含量的增加而增加。相比之下，含磺酸的基于PEO的聚合物上的血小板和纤维蛋白原沉积大大增强。
