Gomila Margarita, Bowien Botho, Falsen Enevold, Moore Edward R B, Lalucat Jorge
Área Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain.
Abt. Molekularphysiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, 37077 Göttingen, Germany.
Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2629-2635. doi: 10.1099/ijs.0.65149-0.
Three Gram-negative, rod-shaped, non-spore-forming bacteria (strains CCUG 52769T, CCUG 52770 and CCUG 52771) isolated from haemodialysis water were characterized taxonomically, together with five strains isolated from industrial waters (CCUG 52428, CCUG 52507, CCUG 52575T, CCUG 52590 and CCUG 52631). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the class Betaproteobacteria and were related to the genus Pelomonas, with 16S rRNA gene sequence similarities higher than 99% with the only species of the genus, Pelomonas saccharophila and to Pseudomonas sp. DSM 2583. The type strains of Mitsuaria chitosanitabida and Roseateles depolymerans were their closest neighbours (97.9 and 97.3% 16S rRNA gene sequence similarity, respectively). Phylogenetic analysis was also performed for the internally transcribed spacer region and for three genes [hoxG (hydrogenase), cbbL/cbbM (Rubisco) and nifH (nitrogenase)] relevant for the metabolism of the genus Pelomonas. DNA-DNA hybridization, major fatty acid composition and phenotypical analyses were carried out, which included the type strain of Pelomonas saccharophila obtained from different culture collections (ATCC 15946T, CCUG 32988T, DSM 654T, IAM 14368T and LMG 2256T), as well as M. chitosanitabida IAM 14711T and R. depolymerans CCUG 52219T. Results of DNA-DNA hybridization, physiological and biochemical tests supported the conclusion that strains CCUG 52769, CCUG 52770 and CCUG 52771 represent a homogeneous phylogenetic and genomic group, including strain DSM 2583, clearly differentiated from the industrial water isolates and from the Pelomonas saccharophila type strain. On the basis of phenotypic and genotypic characteristics, these strains belong to two novel species within the genus Pelomonas, for which the names Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are proposed. The type strains of Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are CCUG 52769T (=CECT 7234T) and CCUG 52575T (=CECT 7233T), respectively.
对从血液透析用水中分离出的3株革兰氏阴性、杆状、无芽孢形成细菌（菌株CCUG 52769T、CCUG 52770和CCUG 52771）以及从工业用水中分离出的5株细菌（CCUG 52428、CCUG 52507、CCUG 52575T、CCUG 52590和CCUG 52631）进行了分类学特征分析。基于16S rRNA基因序列的系统发育分析表明，这些分离株属于β-变形菌纲，与Pelomonas属相关，与Pelomonas属唯一物种嗜糖Pelomonas saccharophila以及假单胞菌属DSM 2583的16S rRNA基因序列相似性高于99%。几丁质脱氨基短杆菌（Mitsuaria chitosanitabida）和解聚玫瑰菌（Roseateles depolymerans）的模式菌株是它们最接近的亲缘菌株（16S rRNA基因序列相似性分别为97.9%和97.3%）。还对内部转录间隔区以及与Pelomonas属代谢相关的3个基因[hoxG（氢化酶）、cbbL/cbbM（核酮糖-1,5-二磷酸羧化酶/加氧酶）和nifH（固氮酶）]进行了系统发育分析。进行了DNA-DNA杂交、主要脂肪酸组成和表型分析，其中包括从不同菌种保藏中心获得的嗜糖Pelomonas saccharophila模式菌株（ATCC 15946T、CCUG 32988T、DSM 654T、IAM 14368T和LMG 2256T），以及几丁质脱氨基短杆菌IAM 14711T和解聚玫瑰菌CCUG 52219T。DNA-DNA杂交、生理和生化试验结果支持以下结论：菌株CCUG 52769、CCUG 52770和CCUG 52771代表一个同质的系统发育和基因组群，包括菌株DSM 2583，明显不同于工业用水分离株和嗜糖Pelomonas saccharophila模式菌株。基于表型和基因型特征，这些菌株属于Pelomonas属内的两个新物种，分别命名为嗜纯水Pelomonas puraquae sp. nov.和水生Pelomonas aquatica sp. nov.。嗜纯水Pelomonas puraquae sp. nov.和水生Pelomonas aquatica sp. nov.的模式菌株分别为CCUG 52769T（=CECT 7234T）和CCUG 52575T（=CECT 7233T）。
Zotero 插件