检测和鉴定生防真菌金龟子绿僵菌 CBS 648.67 和 CFCC 80795 菌株中内共生的新型胞内细菌
Detection and Identification of Novel Intracellular Bacteria Hosted in Strains CBS 648.67 and CFCC 80795 of Biocontrol Fungi Metarhizium.
机构信息
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry.
Beijing Floriculture Engineering Technology Research Centre, Beijing Botanical Garden.
出版信息
Microbes Environ. 2022;37(2). doi: 10.1264/jsme2.ME21059.
"Endosymbiosis" is a cohesive form of a symbiotic association. Endobacteria exist in many fungi and play important roles in fungal host biology. Metarhizium spp. are important entomopathogenic fungi for insect pest control. In the present study, we performed comprehensive ana-lyses of strains of Metarhizium bibionidarum and M. anisopliae using PCR, phylogenetics, and fluorescent electron microscopy to identify endobacteria within hyphae and conidia. The results of the phylogenetic ana-lysis based on 16S rRNA gene sequences indicated that these endobacteria were the most closely related to Pelomonas puraquae and affiliated with Betaproteobacteria. Ultrastructural observations indicated that endobacteria were coccoid and less than 500 nm in diameter. The basic characteristics of endobacteria in M. bibionidarum and M. anisopliae were elucidated, and biological questions were raised regarding their biological functions in the Metarhizium hosts.
“内共生”是一种共生关系的凝聚形式。内生菌存在于许多真菌中，在真菌宿主生物学中发挥着重要作用。金龟子绿僵菌是一种重要的昆虫病原真菌，可用于防治害虫。在本研究中，我们使用 PCR、系统发育分析和荧光电子显微镜对粘帚霉和绿僵菌菌株进行了综合分析，以鉴定菌丝和孢子中的内生菌。基于 16S rRNA 基因序列的系统发育分析结果表明，这些内生菌与 Pelomonas puraquae 最为密切相关，属于β变形菌门。超微结构观察表明，内生菌呈球形，直径小于 500nm。阐述了粘帚霉和绿僵菌内生菌的基本特征，并提出了关于它们在绿僵菌宿主中的生物学功能的生物学问题。
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