Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea.
Antonie Van Leeuwenhoek. 2024 Jun 4;117(1):87. doi: 10.1007/s10482-024-01988-4.
Two novel Gram-stain-negative strains designated P7 and P8, were isolated from the soil of a paddy field in Goyang, Republic of Korea, and identified as new species within the genus Roseateles through a polyphasic taxonomic approach. These aerobic, rod-shaped, non-sporulating strains demonstrated optimal growth at 30 °C, pH 7, and in the absence of NaCl (0% w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated close relationships with Roseateles saccharophilus DSM654 (98.7%) and Roseateles puraquae CCUG 52769 (98.96%), respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the isolates with the most closely related strains with publicly available whole genomes were 82.0-85.5% and 25.0-30.2%, respectively. The predominant fatty acids identified were C and summed feature 3 (composed of C ω6c and/or C ω7c), with minor amounts of C, C 3-OH and summed feature 8 (composed of C ω7c and/or C ω6c; 26.4%). Ubiquinone 8 was the main quinone, and the polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, two unidentified phosphoaminolipids, one unidentified phosphoglycolipid, three unidentified phospholipids, and one unidentified aminolipid. The draft genome sequences revealed genomic DNA G + C contents of 70.1% for P7 and 68.2% for P8. Comprehensive physiological, biochemical, and 16S rRNA sequence analyses confirm these isolates as novel species of the genus Roseateles, proposed to be named Roseateles caseinilyticus sp. nov for strain P7 (= KACC 22504 = TBRC 15694) and Roseateles cellulosilyticus sp. nov. for strain P8 (= KACC 22505 = TBRC 15695).
两株新型革兰氏阴性菌株 P7 和 P8 是从韩国高阳稻田土壤中分离得到的，通过多相分类学方法鉴定为玫瑰杆菌属的新种。这些好氧、杆状、非孢子形成的菌株在 30°C、pH7 和无 NaCl（0%w/v）的条件下表现出最佳生长。基于 16S rRNA 基因序列的系统发育分析表明，它们与 Roseateles saccharophilus DSM654（98.7%）和 Roseateles puraquae CCUG 52769（98.96%）密切相关。与具有公开全基因组的最密切相关菌株的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 82.0-85.5%和 25.0-30.2%。鉴定出的主要脂肪酸为 C 和总和特征 3（由 C ω6c 和/或 C ω7c 组成），少量 C、C 3-OH 和总和特征 8（由 C ω7c 和/或 C ω6c 组成；26.4%）。泛醌 8 是主要的醌，极性脂类谱包括磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的磷酸氨基脂类、一种未鉴定的磷酸糖脂类、三种未鉴定的磷脂类和一种未鉴定的氨基脂类。基因组 DNA G+C 含量分别为 70.1%的 P7 和 68.2%的 P8。综合生理生化和 16S rRNA 序列分析证实这些分离株为玫瑰杆菌属的新种，建议将 P7 菌株（=KACC 22504=TBRC 15694）命名为酪蛋白水解玫瑰杆菌，将 P8 菌株（=KACC 22505=TBRC 15695）命名为纤维素水解玫瑰杆菌。
