Nedashkovskaya Olga I, Kim Seung Bum, Lysenko Anatoly M, Lee Kang Hyun, Bae Kyung Sook, Mikhailov Valery V
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.
Department of Microbiology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2655-2659. doi: 10.1099/ijs.0.65251-0.
Two marine, heterotrophic, aerobic, pigmented and gliding bacteria, isolated from the sea urchin Strongylocentrotus intermedius were subjected to a polyphasic taxonomy study. The 16S rRNA gene sequence analysis revealed that strains KMM 6032T and KMM 6047 formed a distinct branch within the genus Arenibacter, a member of the family Flavobacteriaceae. The level of sequence similarity between the novel isolates and members of the genus Arenibacter was 94.5-98.9%. The DNA G+C content was 39-40 mol%. The dominant fatty acids were iso-C15:1, iso-C15:0, C15:0, C15:1omega6c, iso-C15:0 3-OH, iso-C17:1omega9c, iso-C17:0 3-OH and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1omega7c). The results of DNA-DNA hybridization experiments supported by phenotypic data indicated that the isolates represent a novel species within the genus Arenibacter, for which the name Arenibacter echinorum sp. nov. is proposed. The type strain is KMM 6032T (=KCTC 22013T=LMG 22574T).
从中间球海胆中分离出的两株海洋、异养、需氧、有色素且能滑行的细菌，进行了多相分类学研究。16S rRNA基因序列分析表明，菌株KMM 6032T和KMM 6047在黄杆菌科成员Arenibacter属内形成了一个独特的分支。新分离菌株与Arenibacter属成员之间的序列相似性水平为94.5 - 98.9%。DNA G + C含量为39 - 40 mol%。主要脂肪酸为异-C15:1、异-C15:0、C15:0、C15:1ω6c、异-C15:0 3-OH、异-C17:1ω9c、异-C17:0 3-OH以及总和特征3（包括异-C15:0 2-OH和/或C16:1ω7c）。DNA - DNA杂交实验结果结合表型数据表明，这些分离菌株代表Arenibacter属内的一个新物种，为此提出名为Arenibacter echinorum sp. nov.的名称。模式菌株为KMM 6032T（=KCTC 22013T = LMG 22574T）。
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