Li An-Zhang, Lin Long-Zhen, Zhang Ming-Xia, Lv Yingying, Zhu Hong-Hui
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2018 Mar;68(3):758-763. doi: 10.1099/ijsem.0.002576. Epub 2018 Jan 29.
A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium, designated as P308H10, was isolated from surface sediment of the Southern Indian Ocean. Growth occurred at 4-36 °C (optimum 20-25 °C), pH 6.0-8.5 (optimum 7.5-8.0) and in the presence of 1-8 % (w/v) NaCl (optimum 2-3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain P308H10 lies within the clade of members of the genus Arenibacter and is closely related to Arenibacterhampyeongensis HP12 (98.0 %), Arenibacterechinorum KMM 6032 (98.4 %), Arenibacterpalladensis LMG 21972 (97.9 %), Arenibactertroitsensis KMM 3674 (97.9 %) and 'Arenibacter algicola' TG409 (98.1 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain P308H10 and the five reference strains were 85.9-80.6 % and 30.2-23.6 %, respectively. The major fatty acids (>10 %) of strain P308H10 were summed feature 3, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0. The major polar lipids comprised phosphatidylethanolamine, five unidentified aminolipids and four unidentified lipids. The only respiratory quinone was menaquinone-6. The genomic DNA G+C content was 38.2 mol%. On the basis of the phenotypic, phylogenetic and chemotaxonomic data presented, strain P308H10 represents a novel species of the genus Arenibacter, for which the name Arenibacter catalasegens sp. nov. is proposed. The type strain is P308H10 (=GDMCC 1.1230=KCTC 52983). An emended description of the genus Arenibacter is also proposed.
从南印度洋表层沉积物中分离出一株革兰氏染色阴性、需氧、不运动的杆状细菌，命名为P308H10。该菌在4-36 °C（最适温度20-25 °C）、pH 6.0-8.5（最适pH 7.5-8.0）以及1-8 %（w/v）NaCl（最适浓度2-3 %）条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株P308H10属于嗜盐杆菌属分支，与平岩嗜盐杆菌HP12（相似度98.0 %）、棘嗜盐杆菌KMM 6032（相似度98.4 %）、钯嗜盐杆菌LMG 21972（相似度97.9 %）、特罗伊茨嗜盐杆菌KMM 3674（相似度97.9 %）以及“嗜盐栖藻杆菌”TG409（相似度98.1 %）密切相关。菌株P308H10与这五株参考菌株之间的平均核苷酸一致性和数字DNA-DNA杂交值分别为85.9-80.6 %和30.2-23.6 %。菌株P308H10的主要脂肪酸（>10 %）为特征性脂肪酸3、异C17 : 0 3-OH、异C15 : 1 G和异C15 : 0。主要极性脂类包括磷脂酰乙醇胺、五种未鉴定的氨基脂类和四种未鉴定的脂类。唯一的呼吸醌为甲基萘醌-6。基因组DNA的G+C含量为38.2 mol%。根据所提供的表型、系统发育和化学分类学数据，菌株P308H10代表嗜盐杆菌属的一个新物种，为此提出名为过氧化氢嗜盐杆菌新种（Arenibacter catalasegens sp. nov.）。模式菌株为P308H10（=GDMCC 1.1230=KCTC 52983）。同时还提出了嗜盐杆菌属的修订描述。
