Nedashkovskaya Olga I, Kim Seung Bum, Vancanneyt Marc, Lysenko Anatoly M, Shin Dong Sung, Park Myung Soo, Lee Kang Hyun, Jung Won Jin, Kalinovskaya Natalia I, Mikhailov Valery V, Bae Kyung Sook, Swings Jean
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.
Department of Microbiology, Chungnam National University, 220 Gung-dong, Yusong, Daejon 305-764, Republic of Korea.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):953-958. doi: 10.1099/ijs.0.64156-0.
The taxonomic position of three novel marine, heterotrophic, pigmented and agarolytic bacteria with gliding motility, isolated from the sea urchin Strongylocentrotus intermedius, was investigated. 16S rRNA gene sequence analysis revealed that strains KMM 6166, KMM 6172T and KMM 6173 are members of the phylum Bacteroidetes; their nearest neighbours were Belliella baltica and Hongiella marincola (similarities of 94.5 and 93.6 %, respectively). The DNA G+C content of the strains was 44-45 mol%. The predominant fatty acids were C15 : 0 iso, C16:1omega5c, C17:1 iso omega9c, C17:0 iso 3-OH and summed feature 3 (C16:1omega7c and/or C15:0 iso 2-OH). The major respiratory quinone was MK-7. Results of molecular experiments supported by phenotypic and chemotaxonomic data enabled the isolates to be classified as representatives of a novel species in a new genus, for which the name Echinicola pacifica gen. nov., sp. nov. is proposed. Echinicola pacifica is the type species of the genus Echinicola, and its type strain is KMM 6172T (=KCTC 12368T=LMG 23350T).
对从中间球海胆中分离出的三株具有滑行运动能力的新型海洋异养、产色素且能分解琼脂的细菌进行了分类地位研究。16S rRNA基因序列分析表明，菌株KMM 6166、KMM 6172T和KMM 6173属于拟杆菌门；它们最接近的亲缘种是波罗的海贝利氏菌和海洋洪氏菌（相似度分别为94.5%和93.6%）。这些菌株的DNA G+C含量为44 - 45 mol%。主要脂肪酸为C15 : 0异戊酸、C16:1ω5c、C17:1异戊酸ω9c、C17:0异戊酸3 - OH以及总和特征3（C16:1ω7c和/或C15:0异戊酸2 - OH）。主要呼吸醌为MK - 7。分子实验结果得到表型和化学分类数据的支持，使得这些分离株能够被归类为一个新属中一个新物种的代表，为此提出了太平洋海胆杆菌属（Echinicola pacifica），新属，新种。太平洋海胆杆菌是海胆杆菌属的模式种，其模式菌株为KMM 6172T（=KCTC 12368T=LMG 23350T）。
