Houot Laetitia, Watnick Paula I
Division of Infectious Disease, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
J Bacteriol. 2008 Jan;190(1):311-20. doi: 10.1128/JB.01410-07. Epub 2007 Nov 2.
Glucose is a universal energy source and a potent inducer of surface colonization for many microbial species. Highly efficient sugar assimilation pathways ensure successful competition for this preferred carbon source. One such pathway is the phosphoenolpyruvate phosphotransferase system (PTS), a multicomponent sugar transport system that phosphorylates the sugar as it enters the cell. Components required for transport of glucose through the PTS include enzyme I, histidine protein, enzyme IIA(Glc), and enzyme IIBC(Glc). In Escherichia coli, components of the PTS fulfill many regulatory roles, including regulation of nutrient scavenging and catabolism, chemotaxis, glycogen utilization, catabolite repression, and inducer exclusion. We previously observed that genes encoding the components of the Vibrio cholerae PTS were coregulated with the vps genes, which are required for synthesis of the biofilm matrix exopolysaccharide. In this work, we identify the PTS components required for transport of glucose and investigate the role of each of these components in regulation of biofilm formation. Our results establish a novel role for the phosphorylated form of enzyme I in specific regulation of biofilm-associated growth. As the PTS is highly conserved among bacteria, the enzyme I regulatory pathway may be relevant to a number of biofilm-based infections.
葡萄糖是一种通用的能量来源，也是许多微生物物种表面定殖的强效诱导剂。高效的糖同化途径确保了对这种优质碳源的成功竞争。其中一条途径是磷酸烯醇式丙酮酸磷酸转移酶系统（PTS），这是一种多组分糖转运系统，在糖进入细胞时将其磷酸化。通过PTS转运葡萄糖所需的组分包括酶I、组氨酸蛋白、酶IIA（Glc）和酶IIBC（Glc）。在大肠杆菌中，PTS的组分发挥着许多调节作用，包括营养清除和分解代谢的调节、趋化性、糖原利用、分解代谢物阻遏和诱导物排除。我们之前观察到，编码霍乱弧菌PTS组分的基因与vps基因共同调控，vps基因是生物膜基质胞外多糖合成所必需的。在这项工作中，我们确定了转运葡萄糖所需的PTS组分，并研究了这些组分各自在生物膜形成调控中的作用。我们的结果确立了酶I的磷酸化形式在生物膜相关生长的特定调控中的新作用。由于PTS在细菌中高度保守，酶I调节途径可能与许多基于生物膜的感染有关。
