Silva Wilson A, Gnjatic Sacha, Ritter Erika, Chua Ramon, Cohen Tzeela, Hsu Melinda, Jungbluth Achim A, Altorki Nasser K, Chen Yao-Tseng, Old Lloyd J, Simpson Andrew J G, Caballero Otavia L
Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Cancer Immun. 2007 Nov 6;7:18.
Identification of genes that are upregulated in tumors, and whose normal expression excludes adult somatic tissues but includes germline and/or embryonic tissues, has resulted in a rich variety of cancer antigens that are attractive targets for cancer vaccine and other therapeutic approaches. In the present study, we extended this approach to include genes strongly and restrictively expressed in the placenta by mining publicly available SAGE and EST databases. We identified a number of genes with high expression in placenta and different cancer types but with relatively restricted expression in normal tissues. The gene with the most distinctive expression pattern was found to be PLAC1, which encodes a putative cell surface protein that is highly expressed in placenta, testis, cancer cell lines and lung tumors. Hence we have designated it CT92. We found by ELISA that PLAC1 is immunogenic in a subset of cancer patients and healthy women. Its physical and expression characteristics render it a potential target for both active and passive cancer immunotherapeutic strategies.
鉴定那些在肿瘤中上调表达，且其正常表达排除成人体细胞组织但包括生殖系和/或胚胎组织的基因，已产生了丰富多样的癌症抗原，这些抗原是癌症疫苗和其他治疗方法的有吸引力的靶点。在本研究中，我们通过挖掘公开可用的SAGE和EST数据库，扩展了这种方法，将在胎盘中强烈且限制性表达的基因纳入其中。我们鉴定出了一些在胎盘和不同癌症类型中高表达但在正常组织中表达相对受限的基因。发现具有最独特表达模式的基因是PLAC1，它编码一种假定的细胞表面蛋白，该蛋白在胎盘、睾丸、癌细胞系和肺肿瘤中高度表达。因此我们将其命名为CT92。我们通过ELISA发现，PLAC1在一部分癌症患者和健康女性中具有免疫原性。其物理和表达特征使其成为主动和被动癌症免疫治疗策略的潜在靶点。
