Rodrigues Ana, Ortega Carlos, Santos Luís, Teixeira Alexandre, Dinis Maria Joáo, Vasconcelos Iponina, Lacerda Jorge, Fonseca Elisa
Serviço de Patologia Clínica do Hospital São José, Lisbon, Portugal.
Lab Hematol. 2007;13(3):85-92. doi: 10.1532/LH96.07007.
The aim of this study is to analyze the reticulocyte parameters (classical and research parameters) acquired by the Beckman Coulter GEN'S blood counter (GEN'S; Brea, CA, USA) to establish the "reticulocyte profile" characteristics of patients with anemia of chronic disease (ACD). The reticulocyte parameters and profile provided by the GEN'S were studied in 38 anemic patients who fulfilled the diagnostic criteria for the ACD, and the results were compared with those of 38 healthy controls in a multivariate statistical analysis using the Student t-test and the receiver operating characteristics (ROC) curve analysis.Statistically significant (P <.05) differences between the 2 population groups were observed in several of the reticulocyte parameters provided by the GEN'S: mean volume of the reticulocyte population (MRV) (high), percentage of high light scatter reticulocytes HLR% (high), mean volume of the whole sphered red cell and reticulocyte population (MSCV) (high) MCV-MSCV (low) with the highest diagnostic value, as measured by the area under the ROC curve (>0.9), for the IRF (high) and the reticulocyte population data: mean channel scatter retics (high) and the mean channel conductivity retics (high). This study establishes the reticulocyte parameters and reticulocyte profile as provided by the GEN'S and characteristic of patients with ACD as compared with normal subjects. This should provide the basis for further studies comparing the reticulocyte profile of patients with ACD with those found in other types of anemia.
本研究旨在分析贝克曼库尔特GEN'S血液分析仪（GEN'S；美国加利福尼亚州布雷亚）获取的网织红细胞参数（经典参数和研究参数），以确定慢性病贫血（ACD）患者的“网织红细胞谱”特征。对38例符合ACD诊断标准的贫血患者的GEN'S提供的网织红细胞参数和谱进行了研究，并在多变量统计分析中使用学生t检验和受试者工作特征（ROC）曲线分析，将结果与38例健康对照者的结果进行比较。在GEN'S提供的几个网织红细胞参数中，观察到两组人群之间存在统计学显著差异（P<.05）：网织红细胞群体平均体积（MRV）（高）、高散射光网织红细胞百分比（HLR%）（高）、整个球形红细胞和网织红细胞群体平均体积（MSCV）（高）、MCV-MSCV（低），其中IRF（高）和网织红细胞群体数据的诊断价值最高，通过ROC曲线下面积（>0.9）测量：平均通道散射网织红细胞（高）和平均通道电导率网织红细胞（高）。本研究确定了GEN'S提供的网织红细胞参数和网织红细胞谱，以及与正常受试者相比ACD患者的特征。这应为进一步比较ACD患者与其他类型贫血患者的网织红细胞谱的研究提供基础。
