Banfi Giuseppe, Mauri Clara, Morelli Benedetto, Di Gaetano Nicola, Malgeri Ursula, Melegati Gianluca
Istituto Galeazzi, via Galeazzi 4, 20161 Milan, Italy.
Clin Chem Lab Med. 2006;44(5):616-22. doi: 10.1515/CCLM.2006.094.
The role of measurement of reticulocytes and their parameters is growing in sports medicine. The use of reticulocyte counts in protocols for evaluating and screening for the suspected abuse of hormones that stimulate the bone marrow is an example. Reticulocytes are also important for evaluation of the performance and general health status of athletes, especially for monitoring therapies and diets. The current availability of fully automated haematological systems that can measure reticulocyte numbers and characteristics (volume, density) increases the potential use of these parameters in laboratory and sports medicine. Few studies have considered the application of these parameters in athletes and a lack of specific reference ranges means that their valid clinical use is difficult.
Using a Coulter LH700 instrument, we measured reticulocyte count (Retics), mean reticulocyte volume (MRV), immature reticulocyte fraction (IRF), and mean sphered cell volume (MSCV) in 106 male professional elite athletes (football and rugby players and skiers). Reference intervals for the athletes were compared with the intervals found for a control group of 73 age-matched males.
We calculated the following reference intervals: 0.30-1.54% for Retics, 93.1-114.8 fL for MRV, 0.18-0.39% for IRF, and 76.8-94.5 fL for MSCV.
No statistically significant differences were observed for Retics, MRV, IRF, and MSCV between elite athletes and controls. Significant differences were observed for haemoglobin (Hb), erythrocytes, haematocrit (Ht), and mean corpuscular volume. Moreover, no statistical differences were observed among different sports, whereas differences were remarked in football and rugby players between the samples drawn before the start of competitive season and the samples drawn during the season, demonstrating that reticulocyte counts and parameters are useful for monitoring sportsmen.
网织红细胞及其参数的测定在运动医学中的作用日益重要。例如，在评估和筛查疑似滥用刺激骨髓的激素的方案中使用网织红细胞计数。网织红细胞对于评估运动员的表现和总体健康状况也很重要，尤其是在监测治疗和饮食方面。目前可获得的能够测量网织红细胞数量和特征（体积、密度）的全自动血液学系统增加了这些参数在实验室和运动医学中的潜在应用。很少有研究考虑这些参数在运动员中的应用，并且缺乏特定的参考范围意味着它们的有效临床应用存在困难。
使用库尔特LH700仪器，我们测量了106名男性职业精英运动员（足球运动员、橄榄球运动员和滑雪运动员）的网织红细胞计数（Retics）、平均网织红细胞体积（MRV）、未成熟网织红细胞比例（IRF）和平均球形细胞体积（MSCV）。将运动员的参考区间与73名年龄匹配的男性对照组的区间进行比较。
我们计算出以下参考区间：Retics为0.30 - 1.54%，MRV为93.1 - 114.8 fL，IRF为0.18 - 0.39%，MSCV为76.8 - 94.5 fL。
精英运动员与对照组之间在Retics、MRV、IRF和MSCV方面未观察到统计学上的显著差异。在血红蛋白（Hb）、红细胞、血细胞比容（Ht）和平均红细胞体积方面观察到显著差异。此外，不同运动项目之间未观察到统计学差异，而在足球和橄榄球运动员中，在赛季开始前采集的样本与赛季期间采集的样本之间存在差异，这表明网织红细胞计数和参数对于监测运动员是有用的。
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