Castel David, Debily Marie-Anne, Pitaval Amandine, Gidrol Xavier
CEA, DSV, DRR, Functional Genomics Department, Evry, France.
Methods Mol Biol. 2007;381:375-84. doi: 10.1007/978-1-59745-303-5_19.
As more genomes are sequenced, challenge of rapidly unraveling the functions of genes was faced. To that end, cell microarrays have been recently described that permit transfection of thousands of nucleic acids in parallel and enable the analysis of phenotypic consequences of such perturbations. As many parameters can influence the efficiency of transfection and consequently protein expression or extinction, some important features in manufacturing cell microarrays for functional exploration of genomes were described.
随着越来越多的基因组被测序，人们面临着快速阐明基因功能的挑战。为此，最近有人描述了细胞微阵列，它可以同时转染数千种核酸，并能够分析此类干扰的表型后果。由于许多参数会影响转染效率，进而影响蛋白质表达或缺失，因此本文描述了制造用于基因组功能探索的细胞微阵列时的一些重要特征。
