Zajac Vladimir, Stevurkova Viola, Matelova Lenka, Ujhazy Eduard
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.
Neuro Endocrinol Lett. 2007 Oct;28(5):591-5.
Bacterial DNA isolated from the intestinal tract of 11 American and 30 Slovak HIV/AIDS patients were analyzed by colony and dot blot hybridization assay for HIV-1 specific sequences. Secondly, PCR using primers specific for the HIV-1 gag, pol and env genes for detection of HIV-1 sequences in these DNA were performed.
Intestinal bacteria DNA of HIV/AIDS patients hybridized in colony and dot blot hybridization assay for HIV-1 specific sequences. PCR products synthesized on specific primers of HIV-1 gag (115 bp), env (142 bp), pol-env (1484) genes were found to be for more than 90% homologous to the corresponding sequence in HIV-1.
Intestinal bacteria of HIV/AIDS patients are bearing sequences for more than 90% identical with those of HIV-1.
通过菌落杂交和斑点杂交试验，对从11名美国和30名斯洛伐克艾滋病毒/艾滋病患者肠道中分离出的细菌DNA进行HIV-1特异性序列分析。其次，使用针对HIV-1 gag、pol和env基因的引物进行PCR，以检测这些DNA中的HIV-1序列。
艾滋病毒/艾滋病患者的肠道细菌DNA在针对HIV-1特异性序列的菌落杂交和斑点杂交试验中出现杂交信号。发现以HIV-1 gag（115 bp）、env（142 bp）、pol-env（1484）基因的特异性引物合成的PCR产物与HIV-1中相应序列的同源性超过90%。
艾滋病毒/艾滋病患者的肠道细菌携带的序列与HIV-1的序列相似度超过90%。
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