Department of Cancer Genetics, Cancer Research Institute, Bratislava, Slovakia.
Med Sci Monit. 2011 Feb 25;17(3):CR154-8. doi: 10.12659/msm.881449.
Bacteria and yeasts isolated from respiratory tracts of 39 Cambodian and 28 Kenyan HIV-positive children were tested for the presence of HIV-1 sequences.
MATERIAL/METHODS: Bacteria and yeasts from the respiratory tract (nose, pharyngeal swabs) were isolated from 39 Cambodian and 28 Kenyan HIV-positive children. Bacterial chromosomal DNA was prepared by standard protocol and by Qiagen kit. The PCR specific for HIV sequences was carried out using HIV-1-specific primers.The analysis was performed by colony and dot-blot hybridization using HIV-1-specific primers which represent gag, pol and env genes of the virus. The sequencing of some PCR products was performed on the ABI 373 DNA Sequencer.
The majority of microbes were characterized as Staphylococcus aureus, Klebsiella pneumoniae, and resp. Candida albicans. In some cases E. coli, Streptococcus pyogenes, Proteus mirabilis and Candida tropicalis were identified. Bacteria of 16 Cambodian (41%) and 8 Kenyan (31%) children were found to be positive in colony and dot-blot DNA hybridization. By the sequencing of PCR products synthesized on the template of patients' bacterial DNA using primers 68;69 for env HIV-1 gene, homology of greater than 90% with HIV-1 isolate HXB2 (HIVHXB2CG) was revealed.
Bacteria and yeasts from the respiratory tract of 41% of Cambodian and 31% of Kenyan HIV-positive children bear HIV-like sequences. The role of bacteria in the HIV disease process is discussed.
从 39 名柬埔寨和 28 名肯尼亚 HIV 阳性儿童的呼吸道中分离出细菌和酵母，并检测其是否存在 HIV-1 序列。
材料/方法：从 39 名柬埔寨和 28 名肯尼亚 HIV 阳性儿童的呼吸道（鼻腔、咽拭子）中分离出细菌和酵母。采用标准方案和 Qiagen 试剂盒制备细菌染色体 DNA。采用 HIV-1 特异性引物进行针对 HIV 序列的 PCR。采用针对病毒 gag、pol 和 env 基因的 HIV-1 特异性引物进行菌落和斑点杂交分析。对一些 PCR 产物进行测序，测序在 ABI 373 DNA 测序仪上进行。
大多数微生物被鉴定为金黄色葡萄球菌、肺炎克雷伯菌和白色念珠菌。在某些情况下，鉴定出大肠杆菌、化脓性链球菌、奇异变形杆菌和热带假丝酵母。16 名柬埔寨（41%）和 8 名肯尼亚（31%）儿童的细菌在菌落和斑点 DNA 杂交中呈阳性。使用引物 68；69 对患者细菌 DNA 模板进行 PCR 产物合成的测序结果表明，与 HIV-1 分离株 HXB2（HIVHXB2CG）的同源性大于 90%。
41%的柬埔寨和 31%的肯尼亚 HIV 阳性儿童的呼吸道细菌和酵母带有 HIV 样序列。讨论了细菌在 HIV 疾病过程中的作用。
