Lakin V V, Belykh A G
Farmakol Toksikol. 1976 Mar-Apr;39(2):212-6.
The process of interaction of ACTH and insulin with plasmatic cellular membranes of the rat's liver and artificial lipid membranes (liposomes) was studied by the method of proper proteinic fluorescence and by using hydrophobic fluorescent probes. This process is shown to be attended by conformative readjustments of the membranous proteins and also by definite structural changes in the lipid phase of the membranes. In its action ACTH involves the hydrophobic layer of the lipid phase to a greater extent than does insulin. A comparison of the data on the effect produced by ACTH and insulin on the plasmatic membranes as against the results obtained with liposomes suggests that for the hormones to display their activity these have first to combine with the specific membranous protein, while a change in the lipid phase comes only as a consequence of such an interaction.
采用适当的蛋白质荧光法并使用疏水荧光探针，研究了促肾上腺皮质激素（ACTH）和胰岛素与大鼠肝脏质膜及人工脂质膜（脂质体）的相互作用过程。结果表明，这一过程伴随着膜蛋白的构象调整以及膜脂质相的特定结构变化。与胰岛素相比，ACTH在作用过程中对脂质相疏水层的影响更大。将ACTH和胰岛素对质膜产生的作用数据与脂质体实验结果进行比较后发现，激素要发挥其活性，首先必须与特定的膜蛋白结合，而脂质相的变化只是这种相互作用的结果。
