Al-Yahyaee Said Ali S, Ganguly Shyam Sundar, Al Kindi Mohammed Nasser, Al-Bahrani Ali Ihassan
Department of Biochemistry, College of Medicine and Health Sciences, Sultan Qaboos University, P.O. Box 35, Al-Khoud 123, Sultanate of Oman.
Hum Biol. 2007 Feb;79(1):93-102. doi: 10.1353/hub.2007.0020.
Apolipoprotein E (APOE) polymorphism is a predictor of interindividual variability in plasma levels of lipids and lipoproteins and a predictor of risk of coronary artery disease (CAD). We studied the relationship between APOE polymorphism and lipid profiles and risk of CAD in Omani dyslipidemic patients. This retrospective study included 244 dyslipidemic patients, of whom 67 had CAD. Fasting blood glucose, lipids, and plasma lipoprotein levels were measured using standard methods, and APOE genotypes were detected by PCR-RFLP. The dyslipidemic patients had the following APOE allele frequencies: APOE2, 0.030; APOE3, 0.894; and APOE4, 0.076. APOE allele frequencies between patients with and without CAD showed no significant differences. Compared to APOE3/3 homozygotes, APOE4 allele patients had higher mean levels of low-density lipoprotein (LDL) cholesterol (p = 0.014), apoB (p = 0.031), lower mean levels of apoA1 (p = 0.043), and a trend of higher mean level of total cholesterol (p = 0.084). Thirty-one percent of patients with CAD had the APOE4 allele compared to 26% with the APOE3 allele, but this difference was not significant. Compared with APOE3/3 homozygotes, patients with the APOE4 allele had 1.3 times higher risk for CAD after ignoring dyslipidemia, but this risk was modified after adjusting for dyslipidemia. In conclusion, among dyslipidemic patients, carriers of APOE4 compared to homozygous carriers of APOE*3 had significantly higher levels of LDL cholesterol and apoB, but no relationship with CAD was found.
载脂蛋白E（APOE）多态性是血浆脂质和脂蛋白个体间变异性的预测指标，也是冠状动脉疾病（CAD）风险的预测指标。我们研究了阿曼血脂异常患者中APOE多态性与血脂谱及CAD风险之间的关系。这项回顾性研究纳入了244例血脂异常患者，其中67例患有CAD。采用标准方法测量空腹血糖、血脂和血浆脂蛋白水平，并通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测APOE基因型。血脂异常患者的APOE等位基因频率如下：APOE2为0.030；APOE3为0.894；APOE4为0.076。有CAD和无CAD患者之间的APOE等位基因频率无显著差异。与APOE3/3纯合子相比，携带APOE4等位基因的患者低密度脂蛋白（LDL）胆固醇平均水平更高（p = 0.014），载脂蛋白B（apoB）水平更高（p = 0.031），载脂蛋白A1（apoA1）平均水平更低（p = 0.043），总胆固醇平均水平有升高趋势（p = 0.084）。31%的CAD患者携带APOE4等位基因，而携带APOE3等位基因的患者为26%，但这种差异不显著。与APOE3/3纯合子相比，携带APOE4等位基因的患者在忽略血脂异常后患CAD的风险高1.3倍，但在调整血脂异常后这种风险有所改变。总之，在血脂异常患者中，与APOE3纯合子携带者相比，APOE*4携带者的LDL胆固醇和apoB水平显著更高，但未发现与CAD有关联。
