Jin Nili, Gilbert Jennifer L, Broaddus Russell R, Demayo Francesco J, Jeong Jae-Wook
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Genesis. 2007 Nov;45(11):716-21. doi: 10.1002/dvg.20348.
Mitogen-inducible gene 6 (Mig-6) is a stress-induced gene that serves as a negative regulator of epidermal growth factor (EGF) signaling and acts as a tumor suppressor. Ablation of Mig-6 results in a significant percentage of embryo lethality as well as abnormalities in multiple tissues. To understand the physiological roles of Mig-6, a conditional null allele, Mig-6(f/f) was generated by introducing LoxP sites that flank exons 2 and 4. The Mig-6(f/f) allele was validated by generating recombined Mig-6(-/-) mice using the Zp3-Cre system. The conditional null allele was confirmed by assaying for Mig-6 gene expression in liver, lung, uterus, and skin. The recombined Mig-6(-/-) mice developed pathological changes, such as degenerative joint diseases and skin hyperplasia similar to the previously reported Mig-6 germline null allele. In addition, these mice also had enlarged uteri with endometrial hyperplasia. In summary, this Mig-6(f/f) mouse is a useful tool for the functional study of the Mig-6 gene in a tissue-specific fashion.
丝裂原诱导基因6（Mig-6）是一种应激诱导基因，作为表皮生长因子（EGF）信号通路的负调节因子，发挥肿瘤抑制作用。Mig-6基因缺失会导致相当比例的胚胎致死以及多种组织出现异常。为了解Mig-6的生理作用，通过在第2和第4外显子两侧引入LoxP位点，构建了条件性无效等位基因Mig-6（f/f）。利用Zp3-Cre系统产生重组的Mig-6（-/-）小鼠，验证了Mig-6（f/f）等位基因。通过检测肝脏、肺、子宫和皮肤中的Mig-6基因表达，证实了条件性无效等位基因。重组的Mig-6（-/-）小鼠出现了病理变化，如退行性关节疾病和皮肤增生，类似于先前报道的Mig-6种系无效等位基因。此外，这些小鼠还出现子宫增大和子宫内膜增生。总之，这种Mig-6（f/f）小鼠是一种以组织特异性方式对Mig-6基因进行功能研究的有用工具。
