仓鼠生物钟中的细胞外一氧化氮信号传导

Extracellular nitric oxide signaling in the hamster biological clock.

作者信息

Plano Santiago A, Agostino Patricia V, Golombek Diego A

机构信息

Laboratorio de Cronobiología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal B1876BXD, Buenos Aires, Argentina.

出版信息

FEBS Lett. 2007 Nov 27;581(28):5500-4. doi: 10.1016/j.febslet.2007.10.058. Epub 2007 Nov 6.

DOI:10.1016/j.febslet.2007.10.058

PMID:17991439

Abstract

Nocturnal light pulses induce phase shifts in circadian rhythms and activate cFos expression in the suprachiasmatic nuclei (SCN). We have studied the role of nitric oxide (NO) in the intercellular communication within the dorsal and ventral portions of the SCN in Syrian hamsters. Administration of the NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide blocked photic phase advances in a dose-dependent manner and inhibited light-induced cFos-ir, without affecting light-induced circadian phase delays. These results suggest that NO may act as an intercellular messenger in the SCN, mediating light-induced phase advances.

摘要

夜间光脉冲会引起昼夜节律的相位偏移，并激活视交叉上核（SCN）中的cFos表达。我们研究了一氧化氮（NO）在叙利亚仓鼠SCN背侧和腹侧部分细胞间通讯中的作用。给予NO清除剂2-苯基-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物以剂量依赖的方式阻断了光诱导的相位提前，并抑制了光诱导的cFos免疫反应性，而不影响光诱导的昼夜节律相位延迟。这些结果表明，NO可能作为SCN中的细胞间信使，介导光诱导的相位提前。