Manganas Louis N, Zhang Xueying, Li Yao, Hazel Raphael D, Smith S David, Wagshul Mark E, Henn Fritz, Benveniste Helene, Djuric Petar M, Enikolopov Grigori, Maletic-Savatic Mirjana
SUNY Stony Brook, Stony Brook, NY 11794, USA.
Science. 2007 Nov 9;318(5852):980-5. doi: 10.1126/science.1147851.
The identification of neural stem and progenitor cells (NPCs) by in vivo brain imaging could have important implications for diagnostic, prognostic, and therapeutic purposes. We describe a metabolic biomarker for the detection and quantification of NPCs in the human brain in vivo. We used proton nuclear magnetic resonance spectroscopy to identify and characterize a biomarker in which NPCs are enriched and demonstrated its use as a reference for monitoring neurogenesis. To detect low concentrations of NPCs in vivo, we developed a signal processing method that enabled the use of magnetic resonance spectroscopy for the analysis of the NPC biomarker in both the rodent brain and the hippocampus of live humans. Our findings thus open the possibility of investigating the role of NPCs and neurogenesis in a wide variety of human brain disorders.
通过体内脑成像鉴定神经干细胞和祖细胞（NPCs）可能对诊断、预后和治疗具有重要意义。我们描述了一种用于在体内检测和定量人脑中NPCs的代谢生物标志物。我们使用质子核磁共振波谱来鉴定和表征一种NPCs富集的生物标志物，并证明了其作为监测神经发生的参考物的用途。为了在体内检测低浓度的NPCs，我们开发了一种信号处理方法，该方法能够使用磁共振波谱分析啮齿动物脑和活体人类海马体中的NPC生物标志物。因此，我们的发现为研究NPCs和神经发生在多种人类脑部疾病中的作用开辟了可能性。
