Han Seung-Kyu, Kim Deok-Woo, Jeong Seong-Ho, Hong Yong-Taek, Woo Hong-Suh, Kim Woo-Kyung, Gottrup Finn
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Ann Plast Surg. 2007 Nov;59(5):532-7. doi: 10.1097/01.sap.0000258983.25728.0b.
Many clinical trials have shown the effectiveness of platelet releasates on diabetic wound healing, but large volumes of blood must be aspirated from patients and a platelet separator is required. This study was undertaken to investigate the potential of blood bank platelet concentrate (BBPC) for accelerating diabetic wound healing. Platelet-derived growth factor-BB (PDGF-BB) contents in BBPC were determined by enzyme-linked immunosorbent assay in vitro, and the in vivo study involved comparing extents of wound healing in BBPC-treated and control groups using diabetic mouse wound models. In the in vitro study, 5.2 +/- 1.2 pg of PDGF-BB was found to be released by 1 million platelets in fresh BBPC, and adding thrombin to BBPC significantly increased the levels of PDGF-BB released. Our in vivo study in diabetic mice revealed that BBPC treatment greatly accelerated wound healing. Our results suggest that BBPC has potential to accelerate the healing of diabetic ulcers.
许多临床试验已表明血小板释放物对糖尿病伤口愈合有效，但必须从患者体内抽取大量血液，且需要血小板分离器。本研究旨在探讨血库血小板浓缩物（BBPC）促进糖尿病伤口愈合的潜力。体外通过酶联免疫吸附测定法测定BBPC中血小板衍生生长因子-BB（PDGF-BB）的含量，体内研究则使用糖尿病小鼠伤口模型比较BBPC治疗组和对照组的伤口愈合程度。在体外研究中，发现新鲜BBPC中每100万个血小板释放5.2±1.2 pg的PDGF-BB，向BBPC中添加凝血酶可显著提高PDGF-BB的释放水平。我们对糖尿病小鼠的体内研究表明，BBPC治疗可大大加速伤口愈合。我们的结果表明，BBPC有加速糖尿病溃疡愈合的潜力。
