Metz Claudia H D, Schröder Anja K, Overbeck Silke, Kahmann Laura, Plümäkers Birgit, Rink Lothar
Institute of Immunology, RWTH Aachen University Hospital, Aachen, Germany.
Nutrition. 2007 Feb;23(2):157-63. doi: 10.1016/j.nut.2006.10.007. Epub 2006 Dec 5.
We examined the influence of zinc on T-helper type 1 (Th1)/T-helper type 2 (Th2) balance in human lymphocytes.
Human peripheral blood mononuclear cells or diluted whole blood were cultured for 8 d in the presence of zinc (30 or 60 microM) or 1 microM of N, N, N', N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) (a zinc-specific chelator). Phytohemagglutinin-induced cytokine release was measured by enzyme-linked immunosorbent assay, and expression of CD56/CD69, CCR4/CD3, and CCR5/CD3 and intracellular labile zinc were detected by flow cytometry.
We found that our in vitro supplementation resulted in an increase of intracellular labile zinc comparable to that of a 7-wk administration of 10 mg of zinc per day in vivo. Zinc triggered interferon-gamma release and impaired interleukin-10 release. Phenotypically, a Th2/Th1 shift could not be confirmed after detecting the Th1-specific chemokine receptor CCR5 or CCR4 for Th2 cells. Surprisingly, we detected a larger amount of CD56+ cells after zinc stimulation, leading us to the conclusion that the amount of interferon-gamma release after zinc supplementation might be attributed to the upregulation of natural killer cells after in vitro zinc supplementation rather than to a Th2/Th1 shift.
We suggest that a nutritional intake of 10 mg of zinc increases the quantity of interferon-gamma-producing natural killer cells and strengthens the immune system against neoplasms and viral infections.
我们研究了锌对人淋巴细胞中1型辅助性T细胞（Th1）/2型辅助性T细胞（Th2）平衡的影响。
将人外周血单个核细胞或稀释的全血在锌（30或60微摩尔）或1微摩尔的N，N，N'，N'-四（2-吡啶甲基）乙二胺（TPEN）（一种锌特异性螯合剂）存在下培养8天。通过酶联免疫吸附测定法测量植物血凝素诱导的细胞因子释放，并通过流式细胞术检测CD56/CD69、CCR4/CD3和CCR5/CD3的表达以及细胞内不稳定锌。
我们发现，我们的体外补充导致细胞内不稳定锌增加，这与体内每天服用10毫克锌7周的效果相当。锌引发γ干扰素释放并损害白细胞介素-10释放。在检测Th2细胞的Th1特异性趋化因子受体CCR5或CCR4后，未能从表型上证实Th2/Th1转变。令人惊讶的是，我们在锌刺激后检测到大量CD56+细胞，这使我们得出结论，锌补充后γ干扰素释放量可能归因于体外锌补充后自然杀伤细胞的上调，而不是Th2/Th1转变。
我们认为，摄入10毫克锌可增加产生γ干扰素的自然杀伤细胞数量，并增强免疫系统抵抗肿瘤和病毒感染的能力。
