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嗜热绿菌中编码香叶基香叶基还原酶的bchP基因的鉴定。

Identification of the bchP gene, encoding geranylgeranyl reductase in Chlorobaculum tepidum.

作者信息

Gomez Maqueo Chew Aline, Frigaard Niels-Ulrik, Bryant Donald A

机构信息

Department of Biochemistry and Molecular Biology, S-235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA.

出版信息

J Bacteriol. 2008 Jan;190(2):747-9. doi: 10.1128/JB.01430-07. Epub 2007 Nov 9.

Abstract

The Chlorobaculum tepidum genome contains two paralogous genes, CT2256 and CT1232, whose products are members of the FixC dehydrogenase superfamily and have sequence similarity to geranylgeranyl reductases. Each gene was insertionally inactivated, and the resulting mutants were characterized. CT2256 encodes geranylgeranyl reductase (BchP); CT1232 is not involved in bacteriochlorophyll or chlorophyll biosynthesis.

摘要

嗜热绿杆菌(Chlorobaculum tepidum)基因组包含两个旁系同源基因CT2256和CT1232,其产物是FixC脱氢酶超家族的成员,并且与香叶基香叶基还原酶具有序列相似性。分别对每个基因进行插入失活,并对产生的突变体进行表征。CT2256编码香叶基香叶基还原酶(BchP);CT1232不参与细菌叶绿素或叶绿素的生物合成。

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