Biological Separations and Mass Spectrometry, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Photosynth Res. 2012 Feb;110(3):153-68. doi: 10.1007/s11120-011-9711-8. Epub 2012 Jan 17.
Chloroflexus aurantiacus J-10-fl is a thermophilic green bacterium, a filamentous anoxygenic phototroph, and the model organism of the phylum Chloroflexi. We applied high-throughput, liquid chromatography-mass spectrometry in a global quantitative proteomics investigation of C. aurantiacus cells grown under oxic (chemoorganoheterotrophically) and anoxic (photoorganoheterotrophically) redox states. Our global analysis identified 13,524 high-confidence peptides that matched to 1,286 annotated proteins, 242 of which were either uniquely identified or significantly increased in abundance under photoheterotrophic culture condition. Fifty-four of the 242 proteins are previously characterized photosynthesis-related proteins, including chlorosome proteins, proteins involved in the bacteriochlorophyll biosynthesis, 3-hydroxypropionate (3-OHP) CO(2) fixation pathway, and components of electron transport chains. The remaining 188 proteins have not previously been reported. Of these, five proteins were found to be encoded by genes from a novel operon and observed only in photoheterotrophically grown cells. These proteins candidates may prove useful in further deciphering the phototrophic physiology of C. aurantiacus and other filamentous anoxygenic phototrophs.
橙氧噬细胞菌 J-10-fl 是一种嗜热的绿色细菌，是丝状的厌氧型光合生物，也是绿弯菌门的模式生物。我们采用高通量、液相色谱-质谱联用技术，对好氧（化能有机异养型）和缺氧（光能有机异养型）条件下橙氧噬细胞菌的细胞进行了全局定量蛋白质组学研究。我们的全局分析鉴定出 13524 个高可信度的肽段，这些肽段匹配到 1286 个注释蛋白，其中 242 个蛋白在光异养培养条件下要么是唯一鉴定到的，要么是丰度显著增加的。242 个蛋白中有 54 个是以前鉴定的与光合作用相关的蛋白，包括菌视紫红质蛋白、参与细菌叶绿素生物合成的蛋白、3-羟基丙酸（3-OHP）CO2 固定途径以及电子传递链的组成部分。其余 188 个蛋白以前没有报道过。在这些蛋白中，有 5 个蛋白被发现是由一个新的操纵子编码的，并且只在光异养生长的细胞中观察到。这些候选蛋白可能有助于进一步解析橙氧噬细胞菌和其他丝状厌氧型光合生物的光合生理学。
