一种用于衣藻启动子分析的新检测方法揭示了热休克元件和TATA框在HSP70A启动子介导的转基因表达激活中的作用。
A new assay for promoter analysis in Chlamydomonas reveals roles for heat shock elements and the TATA box in HSP70A promoter-mediated activation of transgene expression.
作者信息
Lodha Mukesh, Schulz-Raffelt Miriam, Schroda Michael
机构信息
Institute of Biology II, Plant Biochemistry, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.
出版信息
Eukaryot Cell. 2008 Jan;7(1):172-6. doi: 10.1128/EC.00055-07. Epub 2007 Nov 9.
Abstract
The aim of this work was to identify cis-regulatory sequences within the Chlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated the HSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat shock elements and the TATA box.
摘要
这项工作的目的是在衣藻热休克蛋白70A（HSP70A）启动子中鉴定顺式调控序列，这些序列介导其对下游启动子表达的刺激作用。为此，我们删除/突变了HSP70A启动子，并使用一种新的检测方法来量化其刺激作用。我们的结果表明，这种作用主要由热休克元件和TATA框介导。
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