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一种用于衣藻启动子分析的新检测方法揭示了热休克元件和TATA框在HSP70A启动子介导的转基因表达激活中的作用。

A new assay for promoter analysis in Chlamydomonas reveals roles for heat shock elements and the TATA box in HSP70A promoter-mediated activation of transgene expression.

作者信息

Lodha Mukesh, Schulz-Raffelt Miriam, Schroda Michael

机构信息

Institute of Biology II, Plant Biochemistry, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.

出版信息

Eukaryot Cell. 2008 Jan;7(1):172-6. doi: 10.1128/EC.00055-07. Epub 2007 Nov 9.

DOI:10.1128/EC.00055-07
PMID:17993574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2224150/
Abstract

The aim of this work was to identify cis-regulatory sequences within the Chlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated the HSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat shock elements and the TATA box.

摘要

这项工作的目的是在衣藻热休克蛋白70A（HSP70A）启动子中鉴定顺式调控序列，这些序列介导其对下游启动子表达的刺激作用。为此，我们删除/突变了HSP70A启动子，并使用一种新的检测方法来量化其刺激作用。我们的结果表明，这种作用主要由热休克元件和TATA框介导。

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Identification of a plastid response element that acts as an enhancer within the Chlamydomonas HSP70A promoter.鉴定一种质体反应元件，其在衣藻HSP70A启动子中作为增强子发挥作用。
Nucleic Acids Res. 2006;34(17):4767-79. doi: 10.1093/nar/gkl602. Epub 2006 Sep 13.
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Analysis of chromatin structure in the control regions of the chlamydomonas HSP70A and RBCS2 genes.衣藻HSP70A基因和RBCS2基因调控区域的染色质结构分析。
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J-domain protein CDJ2 and HSP70B are a plastidic chaperone pair that interacts with vesicle-inducing protein in plastids 1.J结构域蛋白CDJ2和热休克蛋白70B是一对质体伴侣蛋白，它们与质体中的囊泡诱导蛋白1相互作用。
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Regulation of the alternative oxidase Aox1 gene in Chlamydomonas reinhardtii. Role of the nitrogen source on the expression of a reporter gene under the control of the Aox1 promoter.莱茵衣藻中交替氧化酶Aox1基因的调控。氮源对Aox1启动子控制下报告基因表达的作用。
Plant Physiol. 2003 Mar;131(3):1418-30. doi: 10.1104/pp.013409.
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Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas.HSP70启动子中的序列元件可抵消衣藻中转基因转录沉默。
Plant J. 2002 Aug;31(4):445-55. doi: 10.1046/j.1365-313x.2002.01371.x.
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Transcriptional enhancers--on/off gene regulation as an adaptation to silencing in higher eukaryotic nuclei.转录增强子——作为对高等真核细胞核沉默的一种适应的基因开/关调控
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Plant J. 2000 Jan;21(2):121-31. doi: 10.1046/j.1365-313x.2000.00652.x.
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A chloroplast-targeted heat shock protein 70 (HSP70) contributes to the photoprotection and repair of photosystem II during and after photoinhibition.一种定位于叶绿体的热休克蛋白70（HSP70）在光抑制期间及之后对光系统II的光保护和修复起作用。
Plant Cell. 1999 Jun;11(6):1165-78. doi: 10.1105/tpc.11.6.1165.