Molecular Biotechnology & Systems Biology, TU Kaiserslautern, Paul-Ehrlich Straße 23, D-67663 Kaiserslautern, Germany.
Cells. 2019 Nov 28;8(12):1534. doi: 10.3390/cells8121534.
is a well-established model system for basic research questions ranging from photosynthesis and organelle biogenesis, to the biology of cilia and basal bodies, to channelrhodopsins and photoreceptors. More recently, has also been recognized as a suitable host for the production of high-value chemicals and high-value recombinant proteins. However, basic and applied research have suffered from the inefficient expression of nuclear transgenes. The combined efforts of the community over the past decades have provided insights into the mechanisms underlying this phenomenon and have resulted in mutant strains defective in some silencing mechanisms. Moreover, many insights have been gained into the parameters that affect nuclear transgene expression, like promoters, introns, codon usage, or terminators. Here I critically review these insights and try to integrate them into design suggestions for the construction of nuclear transgenes that are to be expressed at high levels.
是一个成熟的模型系统，可用于研究从光合作用和细胞器发生，到纤毛和基体的生物学，再到通道视紫红质和光感受器等基础科学问题。最近，它也被认为是生产高价值化学品和高价值重组蛋白的合适宿主。然而，基础研究和应用研究一直受到核转基因表达效率低下的困扰。过去几十年来，社区的共同努力提供了对这一现象背后机制的深入了解，并产生了一些沉默机制缺陷的突变株。此外，人们还深入了解了影响核转基因表达的参数，如启动子、内含子、密码子使用或终止子。在这里，我批判性地回顾了这些见解，并试图将它们整合到构建拟高表达核转基因的设计建议中。
