Wheeler David, Bhagwat Medha
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, USA.
Methods Mol Biol. 2007;395:149-76.
The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on the BLAST Quick Start mini-course (www.ncbi.nlm.nih.gov/Class/minicourses). In each example, emphasis is placed on practical step-by-step procedures, although relevant theory is also given where it affects the choice of BLAST program, parameters, and database.
基本局部比对搜索工具（BLAST）可查找蛋白质或核苷酸序列之间的局部相似性区域。该程序将核苷酸或蛋白质序列与数据库中的序列进行比较，并计算匹配的统计学显著性。本章首先介绍BLAST，然后基于BLAST快速入门迷你课程（www.ncbi.nlm.nih.gov/Class/minicourses）描述不同BLAST程序的实际应用。在每个示例中，重点是实际的逐步操作步骤，不过在影响BLAST程序、参数和数据库选择的地方也会给出相关理论。
