用于分析生物样品中微小RNA表达的多重检测方法。
Multiplexed detection methods for profiling microRNA expression in biological samples.
作者信息
Wark Alastair W, Lee Hye Jin, Corn Robert M
机构信息
Department of Chemistry, University of California-Irvine, Irvine, CA 92697, USA.
出版信息
Angew Chem Int Ed Engl. 2008;47(4):644-52. doi: 10.1002/anie.200702450.
Abstract
The recent discovery of short, non-protein coding RNA molecules, such as microRNA molecules (miRNAs), that can control gene expression has unveiled a whole new layer of complexity in the regulation of cell function. Since 2001, there has been a surge of interest in understanding the regulatory role of the hundreds to thousands of miRNAs expressed in both plants and animals. Significant progress in this area requires the development of quantitative bioanalytical methods for the rapid, multiplexed detection of all miRNAs that are present in a particular cell or tissue sample. In this Minireview, we discuss some of the latest methods for high-throughput miRNA profiling and the unique technological challenges that must be surmounted in this endeavor.
摘要
最近发现的短链非蛋白质编码RNA分子，如微小RNA分子（miRNA），能够控制基因表达，这揭示了细胞功能调控中全新的复杂层面。自2001年以来，人们对了解动植物中表达的数百至数千种miRNA的调控作用兴趣激增。该领域的重大进展需要开发定量生物分析方法，以便对特定细胞或组织样本中存在的所有miRNA进行快速、多重检测。在这篇小型综述中，我们讨论了一些用于高通量miRNA分析的最新方法以及在此过程中必须克服的独特技术挑战。
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