鉴定卡瓦中C-糖苷类黄酮为潜在诱变化合物。

Identification of C-glycoside flavonoids as potential mutagenic compounds in kava.

作者信息

Jhoo J-W, Ang C Y W, Heinze T M, Deck J, Schnackenberg L K, Beger R D, Dragull K, Tang C-S

机构信息

Natl. Center for Toxicological Research, U.S. Food and Drug Administration, 3900 NCTR Rd., Jefferson, AR 72079, USA.

出版信息

J Food Sci. 2007 Mar;72(2):C120-5. doi: 10.1111/j.1750-3841.2007.00278.x.

DOI:10.1111/j.1750-3841.2007.00278.x

PMID:17995826

Abstract

Kava (Piper methysticum) extract products have been implicated in a number of severe hepatotoxicity cases. However, systematic toxicological studies regarding kava consumption have not been reported. In this study, 6 major kavalactones and different solvent fractions of kava roots, leaves, and stem peelings were evaluated for their mutagenic potential. None of the kavalactones was found to be positive in the experimental concentration ranges tested by the umu test (a sensitive test for point mutations). However, among the different solvent fractions, the n-butanol fraction of kava leaves was positive. Further investigations using bioassay-directed isolation and analysis indicated that 2 C-glycoside flavonoid compounds accounted for the positive mutagenic results. Two isolated compounds were identified as 2''-O-rhamnosylvitexin and schaftoside by NMR and MS techniques.

摘要

卡瓦（Piper methysticum）提取物产品与多起严重肝毒性病例有关。然而，尚未有关于卡瓦消费的系统毒理学研究报告。在本研究中，对卡瓦根、叶和茎皮的6种主要卡瓦内酯及不同溶剂提取物的致突变潜力进行了评估。在所测试的实验浓度范围内，通过umu试验（一种用于点突变的灵敏试验）未发现任何卡瓦内酯呈阳性。然而，在不同的溶剂提取物中，卡瓦叶的正丁醇提取物呈阳性。使用生物测定导向的分离和分析进行的进一步研究表明，两种C-糖苷黄酮类化合物导致了阳性致突变结果。通过核磁共振（NMR）和质谱（MS）技术将两种分离出的化合物鉴定为2''-O-鼠李糖基牡荆素和schaftoside。