番茄红素可抑制脂多糖诱导的小鼠巨噬细胞中促炎介质诱导型一氧化氮合酶的产生。

Lycopene inhibits LPS-induced proinflammatory mediator inducible nitric oxide synthase in mouse macrophage cells.

作者信息

Rafi Mohamed M, Yadav Prem Narayan, Reyes Marynell

机构信息

Dept. of Food Science, Cook College, Rutgers, The State Univ. of New Jersey, 65 Dudley Rd., New Brunswick, NJ 08901, USA.

出版信息

J Food Sci. 2007 Jan;72(1):S069-74. doi: 10.1111/j.1750-3841.2006.00219.x.

DOI:10.1111/j.1750-3841.2006.00219.x

PMID:17995901

Abstract

Lycopene is a fat-soluble red-orange carotenoid found primarily in tomatoes and tomato-derived products, including tomato sauce, tomato paste, and ketchup, and other dietary sources, including dried apricots, guava, watermelon, papaya, and pink grapefruit. In this study, we have demonstrated the molecular mechanism underlying the anti-inflammatory properties of lycopene using a mouse macrophage cell line (RAW 264.7). Treatment with lycopene (10 microM) inhibited lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production (40% compared with the control). Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that lycopene treatment decreased LPS-induced inducible nitric oxide synthase (iNOS) protein and mRNA expression in RAW 264.7 cells, respectively. These results suggest that lycopene has anti-inflammatory activity by inhibiting iNOS proteins and mRNA expressions in mouse macrophage cell lines. Furthermore, cyclooxygenase-2 (COX-2) protein and mRNA expression were not affected by treatment with lycopene.

摘要

番茄红素是一种脂溶性红橙色类胡萝卜素，主要存在于番茄及番茄制品中，包括番茄酱、番茄糊和番茄酱，以及其他饮食来源，如干杏、番石榴、西瓜、木瓜和粉红葡萄柚。在本研究中，我们使用小鼠巨噬细胞系（RAW 264.7）证明了番茄红素抗炎特性的分子机制。用番茄红素（10 microM）处理可抑制脂多糖（LPS）刺激的一氧化氮（NO）产生（与对照组相比降低40%）。蛋白质印迹法和逆转录-聚合酶链反应（RT-PCR）分析表明，番茄红素处理分别降低了RAW 264.7细胞中LPS诱导的诱导型一氧化氮合酶（iNOS）蛋白和mRNA表达。这些结果表明，番茄红素通过抑制小鼠巨噬细胞系中的iNOS蛋白和mRNA表达而具有抗炎活性。此外，环氧合酶-2（COX-2）蛋白和mRNA表达不受番茄红素处理的影响。