Varela Everton Luiz Pompeu, Gomes Antônio Rafael Quadros, da Silva Barbosa Dos Santos Aline, Dos Santos Guimarães Mariana, de Carvalho Eliete Pereira, Ferreira Oberdan Oliveira, de Oliveira Mozaniel Santana, Iriti Marcello, de Aguiar Andrade Eloisa Helena, Percário Sandro
Oxidative Stress Research Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil.
Postgraduate Program in Biodiversity and Biotechnology - BIONORTE Network, Federal University of Pará, Belém, Pará, Brazil.
Parasite Immunol. 2025 Aug;47(8):e70019. doi: 10.1111/pim.70019.
The severity of malaria is associated with low antioxidant availability and elevated free radical production, which induces oxidative damage in cerebral and pulmonary microcirculation. This can be mitigated by dietary antioxidants. We investigated the protective effects of lycopene (LYC) against oxidative changes induced by Plasmodium berghei (Pb). Mice were infected by intraperitoneal injection of 10 parasitized red blood cells and treated orally with LYC (3.11 mg/kg bw/day) or N-acetylcysteine (NAC, 62 mg/kg bw/day). Evaluations were conducted at 1-, 4-, 8- and 12-days post-infection. We measured thiobarbituric acid reactive substances (TBARS), antioxidant capacity by ABTS (AC-ABTS) and DPPH (AC-DPPH) inhibition, uric acid (UA) and nitric oxide (NO) in brain and lung tissues. Infection led to elevated TBARS, AC-ABTS, AC-DPPH, UA and NO, resulting in animal mortality. LYC significantly attenuated the infection-induced increases in TBARS, UA and NO levels compared to Pb (p < 0.0001) and NAC + Pb groups (p < 0.0001) normalising them to Sham levels. These findings highlight LYC's therapeutic potential against malaria-related oxidative stress.
疟疾的严重程度与抗氧化剂可用性低和自由基产生增加有关，这会导致脑和肺微循环中的氧化损伤。膳食抗氧化剂可以减轻这种损伤。我们研究了番茄红素（LYC）对伯氏疟原虫（Pb）诱导的氧化变化的保护作用。通过腹腔注射10个寄生红细胞感染小鼠，并分别口服LYC（3.11毫克/千克体重/天）或N-乙酰半胱氨酸（NAC，62毫克/千克体重/天）进行治疗。在感染后1天、4天、8天和12天进行评估。我们测量了脑和肺组织中的硫代巴比妥酸反应物质（TBARS）、通过ABTS法测定的抗氧化能力（AC-ABTS）和通过DPPH法测定的抗氧化能力（AC-DPPH）、尿酸（UA）和一氧化氮（NO）。感染导致TBARS、AC-ABTS、AC-DPPH、UA和NO升高，从而导致动物死亡。与Pb组（p < 0.0001）和NAC + Pb组（p < 0.0001）相比，LYC显著减轻了感染诱导的TBARS、UA和NO水平的升高，使其恢复到假手术组水平。这些发现突出了LYC对疟疾相关氧化应激的治疗潜力。
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