Stevens Matthew P, Garland Suzanne M, Tabrizi Sepehr N
Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia.
J Virol Methods. 2008 Feb;147(2):290-6. doi: 10.1016/j.jviromet.2007.09.018. Epub 2007 Nov 8.
The recently released Linear Array human papillomavirus (HPV) genotyping test (Roche Diagnostics) provides a standardized method for simultaneous detection of up to 37 individual HPV types. This test offers a rapid approach for detecting and longitudinal monitoring of patients infected with high-risk (HR) HPV. However, it cannot rule out HPV52 infections in the presence of carcinogenic HPV genotypes 33, 35 and/or 58. As such, often only a non-definitive result of HPV52 presence can be reported. This study describes the development of a real-time PCR assay using an HPV52-specific hydrolysis probe in conjunction with the newly released Roche LightCycler 480 system. HPV52 was readily detected among DNA extracts from samples previously identified with possible HPV52 by the linear array test. Specificity was analyzed using a panel of DNA extracts previously identified as containing single/multiple HPV types, with or without HPV52. This is a rapid and simple assay, which could be used as a supplementary test to the Linear Array, to verify the presence or absence of HPV52 among samples testing positive for either HPV33, 35, and/or 58. Laboratories already using the Linear Array HPV genotyping test could adopt this method once internally validated.
最近发布的线性阵列人乳头瘤病毒（HPV）基因分型检测（罗氏诊断公司）提供了一种标准化方法，可同时检测多达37种HPV亚型。该检测为高危（HR）HPV感染患者的检测及纵向监测提供了一种快速方法。然而，在存在致癌性HPV基因型33、35和/或58时，它无法排除HPV52感染。因此，通常只能报告HPV52存在的不确定结果。本研究描述了一种实时PCR检测方法的开发，该方法使用HPV52特异性水解探针并结合新发布的罗氏LightCycler 480系统。在先前通过线性阵列检测确定可能含有HPV52的样本的DNA提取物中，很容易检测到HPV52。使用一组先前确定含有单一/多种HPV亚型（有或无HPV52）的DNA提取物分析特异性。这是一种快速且简单的检测方法，可作为线性阵列检测的补充检测，以验证在HPV33、35和/或58检测呈阳性的样本中是否存在HPV52。已经使用线性阵列HPV基因分型检测的实验室一旦内部验证通过，即可采用此方法。
