中国仓鼠脑内A粒子的5'长末端重复序列完整核苷酸序列。

5' LTR complete nucleotide sequence of Chinese hamster intracisternal A-particle.

作者信息

Servenay M, Kupiec J J, Peries J, Emanoil-Ravier R

机构信息

UPR A0043 Rétrovirus et Rétrotransposons des Vertébrés, Hôpital Saint-Louis, Paris, France.

出版信息

DNA Seq. 1991;1(5):365-7. doi: 10.3109/10425179109020791.

DOI:10.3109/10425179109020791

PMID:1799684

Abstract

Retrovirus like sequences homologous to mouse IAP are present in Chinese hamster genome (Lueders K.K. and Kuff, E.L., 1981, 1983, Servenay et al., 1990). Murine IAP long terminal repeats (LTRs) can function as effective promoters in different cell types (Horowitz M. et al., 1984, Howe, C.C. et al., 1986). Thus CHO IAP sequences could act as retrotransposons in the cellular genome, and in this way affect the expression of other genes at the target sites. We had sequenced previously a Chinese hamster IAP genomic region corresponding mainly to the gag gene and including 57 nucleotides of U5 5' LTR (Servenay et al., 1988). In this paper, we present the 5' LTR complete nucleotide sequence of the Chinese hamster IAP element and its comparison with those of mouse and Syrian hamster.

摘要

与小鼠IAP同源的逆转录病毒样序列存在于中国仓鼠基因组中（Lueders K.K.和Kuff, E.L., 1981, 1983；Servenay等人，1990）。鼠类IAP长末端重复序列（LTRs）在不同细胞类型中可作为有效的启动子（Horowitz M.等人，1984；Howe, C.C.等人，1986）。因此，中国仓鼠IAP序列可作为细胞基因组中的逆转座子，进而影响靶位点上其他基因的表达。我们之前已对中国仓鼠IAP基因组区域进行了测序，该区域主要对应gag基因，并包括57个核苷酸的U5 5' LTR（Servenay等人，1988）。在本文中，我们呈现了中国仓鼠IAP元件的5' LTR完整核苷酸序列，并将其与小鼠和叙利亚仓鼠的序列进行比较。