叙利亚仓鼠基因组中脑内A颗粒基因的长末端重复序列：将苯丙氨酸转运RNA鉴定为假定的引物转运RNA。
Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNAPhe as a putative primer tRNA.
作者信息
Ono M, Ohishi H
出版信息
Nucleic Acids Res. 1983 Oct 25;11(20):7169-79. doi: 10.1093/nar/11.20.7169.
We have determined the nucleotide sequences of long terminal repeat (LTR) regions of Syrian hamster intracisternal A particle (IAP) genes. The size of the LTRs was 350 base-pairs (bp) and 376 bp in two clones, H10 and H18, respectively. Two LTRs at both ends of the IAP gene were linked to directly repeating 6 bp hamster sequences. Many structural features common to the integrated retroviral LTRs such as "CAT" box, "TATAA" box, polyadenylation signal, and terminal inverted repeat (3 bp), were present on each LTR. The estimated length of R region (about 60 bp) was similar to that of the murine leukemia-sarcoma virus. In contrast, the calculated U5 region of 54 bp was the shortest among those of the retroviruses so far studied. Furthermore, from the analysis of primer binding sites, phenylalanine tRNA was for the first time identified as a presumed primer tRNA for reverse transcription. These results clearly distinguish Syrian hamster IAP LTRs from other retroviral ones. Based on the comparison of the sequences between Syrian hamster and laboratory mouse LTRs, the structural features peculiar to the IAP LTRs and the origin of the IAP genes are discussed.
我们已经确定了叙利亚仓鼠脑内A颗粒（IAP）基因长末端重复序列（LTR）区域的核苷酸序列。在两个克隆H10和H18中，LTR的大小分别为350个碱基对（bp）和376 bp。IAP基因两端的两个LTR与直接重复的6 bp仓鼠序列相连。每个LTR上都存在许多与整合逆转录病毒LTR共有的结构特征，如“CAT”盒、“TATAA”盒、多聚腺苷酸化信号和末端反向重复序列（3 bp）。R区域的估计长度（约60 bp）与鼠白血病肉瘤病毒的相似。相比之下，计算得出的54 bp的U5区域是迄今为止研究的逆转录病毒中最短的。此外，通过对引物结合位点的分析，首次鉴定出苯丙氨酸tRNA是推测的逆转录引物tRNA。这些结果清楚地将叙利亚仓鼠IAP LTR与其他逆转录病毒的LTR区分开来。基于叙利亚仓鼠和实验室小鼠LTR序列的比较，讨论了IAP LTR特有的结构特征和IAP基因的起源。
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