Nordhoff Marcel, Rühe Bärbel, Kellermeier Claudia, Moter Annette, Schmitz Rose, Brunnberg Leo, Wieler Lothar H
Institut für Mikrobiologie und Tierseuchen, FU Berlin, Germany.
Vet Microbiol. 2008 Mar 18;127(3-4):334-42. doi: 10.1016/j.vetmic.2007.09.011. Epub 2007 Sep 29.
To evaluate the association of oral Treponema (T.) spp. with severity of canine periodontitis, subgingival plaque samples of dogs of various breeds undergoing surgery were investigated. A wide range of oral Treponema spp. was analysed by a molecular and culture-independent approach applying DNA-DNA dot blot hybridization analysis and fluorescence in situ hybridization using Treponema specific oligonucleotide probes specific for phylogenetic groups I-VII of oral treponemes as well as probes specific for T. socranskii and T. denticola. To assess the periodontal status of affected dogs clinical parameters were measured and the periodontal status was classified from grade 0 (physiological periodont) to 3 (severe periodontitis). The periodontal status correlated significantly with an increasing concentration of volatile sulfur compounds (VSC, r=0.854) determined with a Halimeter, indicating a positive correlation between the presence of VSC-producing bacteria and periodontitis. In this study Treponema spp. of phylogenetic groups III, V-VII were not detected in any sample, whereas T. denticola-like treponemes were found only in 2 of 51 animals. However, treponemes belonging to phylogenetic groups I, II and IV of oral treponemes or T. socranskii were found in up to 64.84% of the dogs. The detection rate of Treponema spp. was significantly associated with an increased periodontal status. Treponemes present in periodontal lesions were also visualized by fluorescence in situ hybridization of gingival biopsies showing Treponema spp. not only in the microbial biofilm but also within the gingival tissue. The data presented here indicate that oral Treponema spp. are associated with canine periodontitis. Similar to human periodontitis, treponemes of groups I, II and IV and T. socranskii were found more frequently the higher the degree of periodontitis was.
为评估口腔密螺旋体属与犬牙周炎严重程度的关联，对接受手术的不同品种犬的龈下菌斑样本进行了调查。采用分子和非培养方法，通过DNA-DNA斑点杂交分析以及使用针对口腔密螺旋体系统发育组I-VII的密螺旋体特异性寡核苷酸探针和针对索氏密螺旋体及齿垢密螺旋体的特异性探针进行荧光原位杂交，分析了多种口腔密螺旋体。为评估患犬的牙周状况，测量了临床参数，并将牙周状况从0级（生理性牙周）分类到3级（重度牙周炎）。牙周状况与用口臭仪测定的挥发性硫化物（VSC）浓度升高显著相关（r=0.854），表明产生VSC的细菌的存在与牙周炎之间存在正相关。在本研究中，任何样本均未检测到系统发育组III、V-VII的密螺旋体属，而齿垢密螺旋体样密螺旋体仅在51只动物中的2只中发现。然而，在高达64.84%的犬中发现了属于口腔密螺旋体系统发育组I、II和IV或索氏密螺旋体的密螺旋体。密螺旋体属的检出率与牙周状况的增加显著相关。通过牙龈活检的荧光原位杂交也可观察到牙周病变中存在的密螺旋体，显示密螺旋体不仅存在于微生物生物膜中，也存在于牙龈组织内。此处呈现的数据表明口腔密螺旋体属与犬牙周炎有关。与人类牙周炎相似，牙周炎程度越高，I、II和IV组密螺旋体及索氏密螺旋体的检出频率越高。
