Riviere G R, Thompson A J, Brannan R D, McCoy D E, Simonson L G
Department of Pediatric Dentistry, Oregon Health Sciences University, Portland 97201-3097, USA.
J Vet Dent. 1996 Dec;13(4):135-8.
Spirochetes have been observed in dental plaque from dogs, but specific spirochetes have not been identified. In particular, it is not known whether treponemes associated with periodontal diseases in humans also occur in dogs, and whether, like in humans, detection of specific treponemes correlates with periodontal status of dogs. Forty-two dogs were grouped according to the worst periodontal condition in the mouth, as determined by overt signs of inflammation and pocket probing depths. A representative specimen of dental plaque was obtained by pooling subgingival plaque collected from three uniform reference sites, irrespective of periodontal status at selected sites. The presence of pathogen-related oral spirochetes. Treponema denticola, and T. socranskii was determined using specific monoclonal antibodies in an immunocytochemical microscopic assay. All three treponemes were detected in all groups, but a significantly greater proportion of dogs with pocket probing depths > or = 5 mm had detectable treponemes, compared to dogs that were in periodontal health.
在狗的牙菌斑中已观察到螺旋体，但尚未鉴定出特定的螺旋体。特别是，尚不清楚与人类牙周疾病相关的密螺旋体是否也存在于狗中，以及是否像在人类中一样，特定密螺旋体的检测与狗的牙周状况相关。根据口腔中最严重的牙周状况，将42只狗分组，牙周状况由炎症的明显迹象和牙周袋探诊深度确定。通过汇集从三个统一参考部位收集的龈下菌斑获得牙菌斑的代表性标本，而不考虑所选部位的牙周状况。使用特异性单克隆抗体通过免疫细胞化学显微镜检测法确定与病原体相关的口腔螺旋体、齿垢密螺旋体和索氏密螺旋体的存在。在所有组中均检测到所有三种密螺旋体，但与牙周健康的狗相比，牙周袋探诊深度≥5mm的狗中可检测到密螺旋体的比例显著更高。
