Tong Fei, Tian Ying-ping, Huo Shu-hua, Hu Lin, Su Jian-ling, Chen Hui, Wang Xia, Liu Li-dou, Gao Heng-bo, Shi Han-wen
Department of Emergency, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Sep;25(9):518-22.
To investigate NF-kappaB activity and the expression of phosphorylated p38 MAPK protein in lung tissue of acute paraquat poisoned rats and the effect of MT.
One hundred and twenty-eight Sprague-Dawley (SD) rats were randomly divided into three experimental groups: poisoned group, MT group and control group. On the 1st, the 3rd, the 7th and the 14th day after exposure, levels of malondialdehyde (MDA) in serum were detected, NF-kappaB activity in the lung tissues was assessed by electrophoresis mobility shift assay (EMSA), the expression of the phosphorylated p38 MAPK was evaluated by Western blot method, the lung pathological changes of rats were observed.
The level of malondialdehyde (MDA) in serum increased significantly in poisoned group on the 1st day (4.45 +/- 1.23), the 3rd day (3.77 +/- 1.12) and the 7th day (2.84 +/- 0.96) nmol/ml compared with that in control group (1.36 +/- 0.52) nmol/ml (P < 0.01). There was a significant decrease in MT group on the 1st day (2.68 +/- 0.85), the 3rd day (1.97 +/- 0.74) and the 7th day (1.53 +/- 0.62) nmol/ml compared with poisoned group (P < 0.05). The expression of the phosphorylated p38 MAPK and NF-kappaB activity in lung tissue of poisoned group significantly increased compared with control group (P < 0.01). There was a significant decrease in NF-kappaB activity and expression of the phosphorylated p38 MAPK in the lung tissues in MT group compared with poisoned group (P < 0.05).
NF-kappaB and p38 MAPK could play an important role in lung injury of poisoned rats. MT may inhibit the expression of NF-kappaB and phosphorylated p38 MAPK, and therefore might have the therapeutical effect on acute paraquat poisoning.
探讨急性百草枯中毒大鼠肺组织中核因子κB（NF-κB）活性及磷酸化p38丝裂原活化蛋白激酶（p38 MAPK）蛋白的表达情况以及褪黑素（MT）的作用。
将128只Sprague-Dawley（SD）大鼠随机分为三个实验组：中毒组、MT组和对照组。在染毒后第1天、第3天、第7天和第14天，检测血清中丙二醛（MDA）水平，采用电泳迁移率变动分析（EMSA）法评估肺组织中NF-κB活性，采用蛋白质印迹法评估磷酸化p38 MAPK的表达，观察大鼠肺组织病理变化。
中毒组血清中丙二醛（MDA）水平在第1天（4.45±1.23）、第3天（3.77±1.12）和第7天（2.84±0.96）nmol/ml时较对照组（1.36±0.52）nmol/ml显著升高（P<0.01）。MT组在第1天（2.68±0.85）、第3天（1.97±0.74）和第7天（1.53±0.62）nmol/ml时较中毒组显著降低（P<0.05）。中毒组肺组织中磷酸化p38 MAPK的表达及NF-κB活性较对照组显著升高（P<0.01）。MT组肺组织中NF-κB活性及磷酸化p38 MAPK的表达较中毒组显著降低（P<0.05）。
NF-κB和p38 MAPK可能在中毒大鼠肺损伤中起重要作用。MT可能抑制NF-κB及磷酸化p38 MAPK的表达，因此可能对急性百草枯中毒有治疗作用。
