一种用于从致病细菌中分离活性核糖体的新型色谱系统。
A novel chromatography system to isolate active ribosomes from pathogenic bacteria.
作者信息
Maguire Bruce A, Wondrack Lillian M, Contillo Leonard G, Xu Zuoyu
机构信息
Department of Exploratory Medicinal Sciences, Pfizer Global Research and Development, Groton Laboratories, Groton, Connecticut 06340, USA.
出版信息
RNA. 2008 Jan;14(1):188-95. doi: 10.1261/rna.692408. Epub 2007 Nov 12.
Abstract
We have developed a novel chromatography for the rapid isolation of active ribosomes from bacteria without the use of harsh conditions or lengthy procedures that damage ribosomes. Ribosomes interact with an alkyl linker attached to the resin, apparently through their RNA component. Examples are given with ribosomes from Escherichia coli, Deinococcus radiodurans, and with clinical isolates of Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA). The ribosomes obtained by this method are unusually intact, so that highly active ribosomes can now be isolated from the clinical isolates, enabling significantly improved in vitro functional assays that will greatly assist the discovery and development of new ribosomally targeted antibiotics.
摘要
我们开发了一种新型色谱法，可从细菌中快速分离出活性核糖体，无需使用会破坏核糖体的苛刻条件或冗长程序。核糖体显然通过其RNA成分与连接在树脂上的烷基接头相互作用。文中给出了来自大肠杆菌、耐辐射球菌的核糖体的例子，以及肺炎链球菌和耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株的例子。通过这种方法获得的核糖体异常完整，因此现在可以从临床分离株中分离出高活性核糖体，从而显著改进体外功能测定，这将极大地有助于发现和开发新的核糖体靶向抗生素。
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