与信使核糖核酸（mRNA）和转运核糖核酸（tRNA）复合的70S核糖体的结构。

Structure of the 70S ribosome complexed with mRNA and tRNA.

作者信息

Selmer Maria, Dunham Christine M, Murphy Frank V, Weixlbaumer Albert, Petry Sabine, Kelley Ann C, Weir John R, Ramakrishnan V

机构信息

MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.

出版信息

Science. 2006 Sep 29;313(5795):1935-42. doi: 10.1126/science.1131127. Epub 2006 Sep 7.

DOI:10.1126/science.1131127

PMID:16959973

Abstract

The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

摘要

细菌70S核糖体的晶体结构精修至2.8埃分辨率，揭示了其与信使核糖核酸（mRNA）和转运核糖核酸（tRNA）相互作用的原子细节。一种金属离子稳定了mRNA中的一个弯折，该弯折划定了A位点和P位点之间的边界，这对防止mRNA滑动可能很重要。金属离子还稳定了亚基间界面。与古细菌核糖体相比，E位点tRNA与50S亚基的相互作用既有相似之处，也有不同之处。该结构也使许多关于翻译的生化和遗传数据变得合理。