Sogin M L, Pace N R
J Biol Chem. 1976 Jun 10;251(11):3480-8.
The complete nucleotide sequence of a 179-nucleotide precursor (p5A) of 5 S ribosomal RNA from Bacillus subtilis is presented. In addition to the 116-nucleotide mature segment, the p5A molecule contains 21 additional nucleotides at its 5' end and 42 at its 3' terminus. Structural features within the p5A molecule which possibly interact with a specific maturation endonuclease (RNase M5) are identifiable. These include 2-fold rotational symmetry about the cleavage sites, which may approximately place the p5A molecule on the RNase M5 surface, and translational (positional) symmetry, which may precisely orient substrate phosphodiester bonds with respect to catalytic amino acids. Certain features of the 3' -terminal, precursor-specific portion of p5A possibly are involved in the termination of DNA transcription.
本文给出了来自枯草芽孢杆菌的5S核糖体RNA的179个核苷酸前体（p5A）的完整核苷酸序列。除了116个核苷酸的成熟片段外，p5A分子在其5'端还含有21个额外的核苷酸，在其3'端含有42个核苷酸。p5A分子内可能与特定成熟内切核酸酶（RNase M5）相互作用的结构特征是可以识别的。这些特征包括围绕切割位点的二重旋转对称性，它可能使p5A分子大致定位在RNase M5表面，以及平移（位置）对称性，它可能使底物磷酸二酯键相对于催化氨基酸精确取向。p5A 3'端前体特异性部分的某些特征可能参与DNA转录的终止。
