Brennan S M, Geiduschek E P
Nucleic Acids Res. 1983 Jun 25;11(12):4157-75. doi: 10.1093/nar/11.12.4157.
We have determined the nucleotide sequences of four termination sites recognized by Bacillus subtilis RNA polymerase. These sites are located in the terminally repeated segment of the bacteriophage SP01 genome, where most early phage transcription occurs. The SP01 terminators have structures that are similar to those recognized by Escherichia coli RNA polymerase, containing a region of dyad symmetry followed by a stretch of HMU residues in the noncoding DNA strand (HMU is substituted for T in SP01 DNA). We note that in a terminator that is only 60% efficient in vitro, there is a greater distance between these two conserved elements that exists in more efficient terminators. We also find that RNA polymerase molecules which elongate a transcript through a partial terminator often pause at this site.
我们已经确定了枯草芽孢杆菌RNA聚合酶识别的四个终止位点的核苷酸序列。这些位点位于噬菌体SP01基因组的末端重复片段中，早期噬菌体转录大多在此处发生。SP01终止子具有与大肠杆菌RNA聚合酶识别的终止子相似的结构，包含一个二重对称区域，随后是非编码DNA链中的一段HMU残基（在SP01 DNA中，HMU替代了T）。我们注意到，在一个体外效率仅为60%的终止子中，这两个保守元件之间的距离比效率更高的终止子中的距离更大。我们还发现，通过部分终止子延伸转录本的RNA聚合酶分子经常在此位点暂停。
