Loughney K, Lund E, Dahlberg J E
Nucleic Acids Res. 1983 Oct 11;11(19):6709-21. doi: 10.1093/nar/11.19.6709.
The DNA sequence of the region corresponding to the 5'-end of a 16S rRNA gene of B. subtilis 168 was determined. Comparison of this sequence with the sequences flanking other 16S and 23S rRNA coding regions (1-4) indicated that large RNA stem structures, surrounding the mature 16S and 23S rRNAs, could form in a precursor rRNA. The 5'-ends of the precursors of 16S and 23S rRNAs (p16S and p23S) were mapped to the middles of these potential RNA stem structures. We propose that the initial cleavages of the primary rRNA transcript occur near the "opposed G's" which interrupt the basepairing of each of these stem structures. This model is supported by the finding that the 5'-end of the 5S rRNA precursor, p5A (5), maps to the region of the "opposed G's" in the 23S rRNA stem structure.
测定了枯草芽孢杆菌168的16S rRNA基因5'端对应区域的DNA序列。将该序列与其他16S和23S rRNA编码区域（1-4）侧翼的序列进行比较，结果表明围绕成熟16S和23S rRNA的大型RNA茎结构可在前体rRNA中形成。16S和23S rRNA前体（p16S和p23S）的5'端定位于这些潜在RNA茎结构的中部。我们提出，初级rRNA转录本的初始切割发生在打断这些茎结构中每一个碱基配对的“相对G”附近。5S rRNA前体p5A（5）的5'端定位于23S rRNA茎结构中“相对G”区域这一发现支持了该模型。
