Jay Gregory D, Racht Justin, McMurdy John, Mathews Zara, Hughes Ashley, Suner Selim, Crawford Gregory
Warren Alpert Medical School of Brown University, RI 02912, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2007;2007:2932-5. doi: 10.1109/IEMBS.2007.4352943.
Rapidly identifying anemia through qualitative observation of the palpebral conjunctiva for pallor is standard medical practice. We report on the ability of using a fiber optic array probe to collect diffusely reflected light from the palpebral conjunctiva and measure hemoglobin concentration noninvasively. Patients (N=102) admitted to the emergency department and receiving a complete blood count (CBC) were enrolled as a convenience set. The tarsal plate was exposed by averting the lower eyelid and illuminated with a white LED while diffusely reflected light was collected using a fiber optic probe and commercial grating spectrometer. Spectra were analyzed using partial least squares regression to extract hemoglobin concentration information and compare to the CBC result. The probe and algorithm demonstrated correlation coefficient of r = 0.82 and standard error of 1.05 g/dL compared to invasive CBC test. Experimental error is due to variability in distance and position of the probe relative to the conjunctiva. This technique has shown the ability to measure total hemoglobin noninvasively and in real-time at the point-of-care using diffuse reflectance spectroscopy of the tarsal plate, leading to potential inclusion of this class of device as a triage centric tool to identify anemia appearing with internal hemorrhage or other acute conditions.
通过定性观察睑结膜苍白来快速识别贫血是标准的医疗实践。我们报告了使用光纤阵列探头从睑结膜收集漫反射光并无创测量血红蛋白浓度的能力。作为便利样本，纳入了入住急诊科且接受全血细胞计数（CBC）的患者（N = 102）。通过翻开下眼睑暴露睑板，用白色发光二极管照明，同时使用光纤探头和商用光栅光谱仪收集漫反射光。使用偏最小二乘回归分析光谱，以提取血红蛋白浓度信息并与CBC结果进行比较。与侵入性CBC测试相比，该探头和算法的相关系数r = 0.82，标准误差为1.05 g/dL。实验误差是由于探头相对于结膜的距离和位置存在变化。这项技术已显示出能够通过睑板的漫反射光谱在护理点无创且实时地测量总血红蛋白，这使得这类设备有可能作为一种以分诊为中心的工具，用于识别因内出血或其他急性病症而出现的贫血。
