Myakov Alexey, Nieman Linda, Wicky Lorenz, Utzinger Urs, Richards-Kortum Rebecca, Sokolov Konstantin
University of Texas, Biomedical Engineering Program, Austin, Texas 78712, USA.
J Biomed Opt. 2002 Jul;7(3):388-97. doi: 10.1117/1.1483314.
We present the design and construction of a fiber optic probe for elastic light scattering spectroscopy in vivo with polarized excitation and polarization sensitive detection. The performance of the fiber probe is evaluated using a suspension of polystyrene spheres placed atop a diffusely scattering substrate, and it demonstrates that the size-dependent characteristics of the scatterers can be extracted in the presence of a highly diffusely scattering background using a linear combination of forward and backward Mie scattering components of the scatterers. Subsequently, Mie theory calculations are performed over a broad range of diagnostically relevant parameters of nuclei-mean diameter, size distribution, and relative refractive index-to understand how the polarized reflectance measurements with the fiber probe can be used to extract morphological information about epithelial tissue. Finally, the feasibility of in vivo measurements with the fiber optic based polarization sensitive light scattering spectroscopy is demonstrated.
我们展示了一种用于体内弹性光散射光谱的光纤探头的设计与构建，该探头具有偏振激发和偏振敏感检测功能。使用置于漫散射基底上的聚苯乙烯球悬浮液对光纤探头的性能进行了评估，结果表明，在存在高度漫散射背景的情况下，利用散射体向前和向后米氏散射分量的线性组合，可以提取散射体的尺寸相关特性。随后，针对细胞核平均直径、尺寸分布和相对折射率等一系列广泛的诊断相关参数进行了米氏理论计算，以了解如何利用光纤探头的偏振反射测量来提取上皮组织的形态学信息。最后，证明了基于光纤的偏振敏感光散射光谱进行体内测量的可行性。
