Cökeliler Dilek, Göktaş Hilal, Tosun Pinar Deniz, Mutlu Selma
Baskent University, Biomedical Engineering Department, Bağlica Campus, 06810, Ankara, Turkey.
J Nanosci Nanotechnol. 2010 Apr;10(4):2583-9. doi: 10.1166/jnn.2010.1414.
As biomedical materials, titanium and titanium alloys (Ti-6Al-4V) are superior to many materials in terms of mechanical properties and biocompatibility. However, they are still not sufficient for prolonged clinical use because the biocompatibility of these materials must be improved. In this study, the prevention of the attachment of test microorganism on the Ti alloy surfaces by thiol (-SH) and hydroxyl (-OH) functional group containing monomer in plasma based electron beam generator was reported in order to prepare anti-fouling surfaces. The precursor, 11-mercaptoundecanoic acid is used as plasma source to create nano-film with 30-60 nm approximately. The surface chemistry and topology of uncoated and coated samples are characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Atomic Force Microscopy (AFM). Static contact angle measurements are performed to state the change of surface hydrophilicity. All coated samples are tested in-vitro environment with Staphylococcus epidermidis that is chosen as the test bacteria strain in view of its significance for the pathogenesis of medical-device-related infections. This test is repeated after certain period of times and samples are waited in dynamic fluid media in order to investigate the stability of nano-coating. Plasma polymerized 11-mercaptoundecanoic acid film (PP MUA) with 42 +/- 4 nm is found alternative, stabile and simple method to create bacterial anti-fouling surfaces. The static contact angle of the coated surface is 34 +/- 80 whereas the uncoated surface is 57 +/- 50. For the coated surface, the presence of C-OH and C==O groups in infrared spectra defining the PP MUA is achieved by the plasma polymerization. The attachment of the model microorganism on the biomaterial surface prepared by PP MUA is reduced 85.3% if compared to unmodified control surface.
作为生物医学材料，钛及钛合金（Ti-6Al-4V）在机械性能和生物相容性方面优于许多材料。然而，由于这些材料的生物相容性仍需提高，它们仍不足以满足长期临床使用的需求。在本研究中，报道了在基于等离子体的电子束发生器中，通过含硫醇（-SH）和羟基（-OH）官能团的单体来防止测试微生物附着在钛合金表面，以制备防污表面。前驱体11-巯基十一烷酸用作等离子体源，以形成厚度约为30-60nm的纳米膜。通过傅里叶变换红外光谱（FTIR）和原子力显微镜（AFM）对未涂层和涂层样品的表面化学和拓扑结构进行表征。进行静态接触角测量以说明表面亲水性的变化。鉴于表皮葡萄球菌对医疗器械相关感染发病机制的重要性，所有涂层样品都在体外环境中用表皮葡萄球菌进行测试。在一定时间后重复此测试，并将样品置于动态流体介质中，以研究纳米涂层的稳定性。发现具有42±4nm的等离子体聚合11-巯基十一烷酸膜（PP MUA）是创建细菌防污表面的替代、稳定且简单的方法。涂层表面的静态接触角为34±8°，而未涂层表面为57±5°。对于涂层表面，通过等离子体聚合在红外光谱中实现了定义PP MUA的C-OH和C=O基团的存在。与未改性的对照表面相比，PP MUA制备的生物材料表面上模型微生物的附着减少了85.3%。
