Kallio P, Syrjänen S, Tervahauta A, Syrjänen K
Department of Pathology, Kuopio University, Finland.
J Virol Methods. 1991 Nov;35(1):39-47. doi: 10.1016/0166-0934(91)90083-c.
A simple method of processing formalin-fixed, paraffin-embedded tissue sections for DNA amplification by polymerase chain reaction (PCR) is described. In this procedure, deparaffinized sections are readily subjected to DNA isolation simply by boiling and the released DNA can be directly employed for PCR. The method allows analysis of single-copy genes or viral sequences at least up to 300 base pairs long in one working day. This method is particularly useful in analysing retrospective materials when the simplicity and low cost of the assay are preferable. Furthermore, the simplicity of the procedure reduces the risk of contamination.
本文描述了一种通过聚合酶链反应（PCR）对福尔马林固定、石蜡包埋组织切片进行DNA扩增的简单方法。在此方法中，脱蜡后的切片只需煮沸即可轻松进行DNA分离，释放出的DNA可直接用于PCR。该方法能够在一个工作日内分析至少长达300个碱基对的单拷贝基因或病毒序列。当检测的简便性和低成本更为可取时，此方法在分析回顾性材料时特别有用。此外，该方法步骤简单，降低了污染风险。
