Meng Xiaolong, Ichim Thomas E, Zhong Jie, Rogers Andrea, Yin Zhenglian, Jackson James, Wang Hao, Ge Wei, Bogin Vladimir, Chan Kyle W, Thébaud Bernard, Riordan Neil H
Bio-Communications Research Institute, Wichita, USA.
J Transl Med. 2007 Nov 15;5:57. doi: 10.1186/1479-5876-5-57.
Angiogenesis is a critical component of the proliferative endometrial phase of the menstrual cycle. Thus, we hypothesized that a stem cell-like population exist and can be isolated from menstrual blood. Mononuclear cells collected from the menstrual blood contained a subpopulation of adherent cells which could be maintained in tissue culture for >68 doublings and retained expression of the markers CD9, CD29, CD41a, CD44, CD59, CD73, CD90 and CD105, without karyotypic abnormalities. Proliferative rate of the cells was significantly higher than control umbilical cord derived mesenchymal stem cells, with doubling occurring every 19.4 hours. These cells, which we termed "Endometrial Regenerative Cells" (ERC) were capable of differentiating into 9 lineages: cardiomyocytic, respiratory epithelial, neurocytic, myocytic, endothelial, pancreatic, hepatic, adipocytic, and osteogenic. Additionally, ERC produced MMP3, MMP10, GM-CSF, angiopoietin-2 and PDGF-BB at 10-100,000 fold higher levels than two control cord blood derived mesenchymal stem cell lines. Given the ease of extraction and pluripotency of this cell population, we propose ERC as a novel alternative to current stem cells sources.
血管生成是月经周期中增殖期子宫内膜的一个关键组成部分。因此，我们推测存在一种干细胞样细胞群，并且可以从月经血中分离出来。从月经血中收集的单核细胞包含一个贴壁细胞亚群，该亚群在组织培养中可维持超过68次倍增，并保留CD9、CD29、CD41a、CD44、CD59、CD73、CD90和CD105等标志物的表达，且无核型异常。这些细胞的增殖率显著高于对照脐带间充质干细胞，每19.4小时倍增一次。我们将这些细胞称为“子宫内膜再生细胞”（ERC），它们能够分化为9种细胞谱系：心肌细胞、呼吸道上皮细胞、神经细胞、肌细胞、内皮细胞、胰腺细胞、肝细胞、脂肪细胞和成骨细胞。此外，ERC产生MMP3、MMP10、GM-CSF、血管生成素-2和PDGF-BB的水平比两种对照脐带血来源的间充质干细胞系高10-100,000倍。鉴于该细胞群提取容易且具有多能性，我们提出ERC可作为当前干细胞来源的一种新型替代物。
