Fujiya Mikihiro, Musch Mark W, Nakagawa Yasushi, Hu Shien, Alverdy John, Kohgo Yutaka, Schneewind Olaf, Jabri Bana, Chang Eugene B
Martin Boyer Laboratories, University of Chicago, Chicago, IL 60637, USA.
Cell Host Microbe. 2007 Jun 14;1(4):299-308. doi: 10.1016/j.chom.2007.05.004.
Bacteria use quorum-sensing molecules (QSMs) to communicate within as well as across species. However, the effects of QSMs on eukaryotic host cells have received limited attention. We report that the quorum-sensing pentapeptide, competence and sporulation factor (CSF), of the Gram-positive bacterium Bacillus subtilis activates key survival pathways, including p38 MAP kinase and protein kinase B (Akt), in intestinal epithelial cells. CSF also induces cytoprotective heat shock proteins (Hsps), which prevent oxidant-induced intestinal epithelial cell injury and loss of barrier function. These effects of CSF depend on its uptake by an apical membrane organic cation transporter-2 (OCTN2). Thus, OCTN2-mediated CSF transport serves as an example of a host-bacterial interaction that allows the host to monitor and respond to changes in the behavior or composition of colonic flora.
细菌利用群体感应分子（QSMs）在种内以及种间进行通讯。然而，QSMs对真核宿主细胞的影响受到的关注有限。我们报告称，革兰氏阳性菌枯草芽孢杆菌的群体感应五肽、感受态和芽孢形成因子（CSF）可激活肠道上皮细胞中的关键生存途径，包括p38丝裂原活化蛋白激酶和蛋白激酶B（Akt）。CSF还可诱导细胞保护性热休克蛋白（Hsps），从而防止氧化剂诱导的肠道上皮细胞损伤和屏障功能丧失。CSF的这些作用取决于其通过顶端膜有机阳离子转运体2（OCTN2）的摄取。因此，OCTN2介导的CSF转运是宿主-细菌相互作用的一个例子，这种相互作用使宿主能够监测并响应结肠菌群行为或组成的变化。
