Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1, Midorigaoka-higashi, Asahikawa, Hokkaido 078-8510, Japan.
Int J Colorectal Dis. 2012 Aug;27(8):1039-46. doi: 10.1007/s00384-012-1416-8. Epub 2012 Feb 3.
Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. These probiotic effects are considered to be displayed through the mediation of effective molecules derived from these bacteria because live bacteria as well as their conditioned media exhibit beneficial effects in many cases. However, many of the probiotic-derived molecules which mediate such benefits have so far been poorly characterized. We previously found that competence and sporulation factor (CSF) activates the Akt and p38 MAPK pathways and protects epithelial cells from oxidant stress in the mammalian intestine. The purpose of this study is to determine the CSF effect on reducing intestinal inflammation.
A protein array demonstrated that CSF induced the anti-inflammatory cytokine, IL-10, and decreased the release of pro-inflammatory mediators, IL-4, IL-6 and CXCL-1, induced by TNF-α in Caco2/bbe cells. CSF also induced the cytoprotective protein Hsp 27 in Caco2/bbe cells. The histological score of intestinal inflammation in 2% dextran sodium sulfate (DSS)-treated mice with the administration of 10 nM CSF was significantly lower than that of control mice. CSF also improved the survival rate of mice treated with a lethal concentration of DSS.
Therefore, CSF is a potentially effective treatment for intestinal inflammation.
益生菌被定义为活的微生物，当以足够的量给药时，对宿主赋予健康益处。这些益生菌作用被认为是通过这些细菌衍生的有效分子的介导来显示的，因为活细菌及其条件培养基在许多情况下都表现出有益的效果。然而，迄今为止，许多介导这些益处的益生菌衍生分子的特征描述很差。我们之前发现，感应和孢子形成因子 (CSF) 可激活 Akt 和 p38 MAPK 途径，并保护哺乳动物肠道中的上皮细胞免受氧化应激。本研究的目的是确定 CSF 对减少肠道炎症的影响。
蛋白质阵列表明，CSF 诱导抗炎细胞因子 IL-10 的释放，并减少 TNF-α诱导的 Caco2/bbe 细胞中促炎介质 IL-4、IL-6 和 CXCL-1 的释放。CSF 还诱导 Caco2/bbe 细胞中的细胞保护蛋白 Hsp27。用 10 nM CSF 处理的 2%葡聚糖硫酸钠 (DSS) 处理的小鼠的肠道炎症组织学评分明显低于对照组小鼠。CSF 还提高了用致死剂量 DSS 处理的小鼠的存活率。
因此，CSF 是一种治疗肠道炎症的有效药物。
