Refik Saydam National Public Health Agency, Ankara, Turkey.
J Clin Virol. 2010 Aug;48(4):264-9. doi: 10.1016/j.jcv.2010.05.011. Epub 2010 Jun 25.
Phleboviruses cause sandfly fever but isolates are rare.
To analyse samples from concurrent outbreaks of suspected sandfly fever in the Mediterranean provinces of Adana, Izmir and the central province of Ankara, Turkey.
Samples from acute cases were analysed by immunofluorescence assay (IFA). Virus isolation was attempted and pyrosequencing performed.
In IFA 38% of 106 samples tested scored IgM positive for sandfly fever Sicillian virus (SFSV), 12% for SFSV/sandfly fever Cyprus Virus (SFCV) and only 4% for SFCV. A sandfly fever Sicilian type virus designated sandfly fever Turkey virus (SFTV) was isolated. The S-segment sequence of SFTV had a homology of 98% to that of SFCV. The M-segment sequence showed a 91.1% homology to the only SFSV sequence available. The L-segment sequence showed a homology of 58% and 60.3% to Toscana virus and Rift Valley Fever virus sequences, a partial 201nt sequence showed 95.5% homology to the SFSV Sabin strain.
A new phlebovirus related to sandfly fever Sicilian virus, SFTV was isolated and characterized from acute patient material. The sandfly fever Sicilian virus activity seems to be changing in Turkey. Entomological studies are needed.
沙蝇热病毒可引起沙蝇热，但分离株较为罕见。
分析土耳其地中海省份阿达纳、伊兹密尔和中部安卡拉同时发生的疑似沙蝇热暴发的样本。
采用免疫荧光分析（IFA）对急性病例样本进行分析。尝试进行病毒分离，并进行焦磷酸测序。
在 IFA 中，106 份检测样本中有 38%的样本对沙蝇热西西里病毒（SFSV）的 IgM 呈阳性，12%的样本对 SFSV/塞浦路斯沙蝇热病毒（SFCV）呈阳性，只有 4%的样本对 SFCV 呈阳性。分离到一种被命名为沙蝇热土耳其病毒（SFTV）的沙蝇热西西里型病毒。SFTV 的 S 片段序列与 SFCV 的同源性为 98%。M 片段序列与唯一可用的 SFSV 序列具有 91.1%的同源性。L 片段序列与托斯卡纳病毒和裂谷热病毒序列的同源性分别为 58%和 60.3%，一段 201nt 的部分序列与 SFSV Sabin 株的同源性为 95.5%。
从急性患者标本中分离并鉴定出一种与沙蝇热西西里病毒相关的新沙蝇热病毒，即 SFTV。沙蝇热西西里病毒在土耳其的活动似乎正在发生变化。需要进行昆虫学研究。
