使用离子对检测法对脂肪族磺酸盐表面活性剂进行高效液相色谱分析。

HPLC analysis of aliphatic sulfonate surfactants using ion-pair detection.

作者信息

Zahrobsky Marek, Camporese Davide, Rist Øystein, Carlsen Per H J

机构信息

Department of Chemistry, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

出版信息

Molecules. 2005 Sep 30;10(9):1179-89. doi: 10.3390/10091179.

DOI:10.3390/10091179

PMID:18007384

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6147533/

Abstract

A method was developed for the analysis of a number of surfactants which contained no UV-chromophores, using RP-HPLC with Indirect Photometric Detection, IPD. Pyridinium salts such as N-methylpyridinium iodide, N-methyl-2,2'-dipyridinium iodide and N,N'-dimethyl-2,2'-dipyridinium diiodide, were used as the visualization reagents, forming ion-pair complexes with the sulfonate surfactants. This allowed ordinary UV-detection. N-methylpyridinium iodide proved to be a suitable reagent, both with respect to ease of preparation and response. The eluents consisted of mixtures of acetonitrile and water, being 0.1 - 0.25 mM with respect to pyridinium salt. The method was sensitive and exhibited good signal to noise ratios, as well as linear responses over a wide concentration range. All of the analyzed surfactants were separated, including the diastereomeric forms of some of the surfactants.

摘要

开发了一种分析多种不含紫外发色团表面活性剂的方法，该方法采用带间接光度检测（IPD）的反相高效液相色谱法。使用吡啶盐，如碘化N - 甲基吡啶、碘化N - 甲基 - 2,2'-联吡啶和二碘化N,N'-二甲基 - 2,2'-联吡啶，作为可视化试剂，与磺酸盐表面活性剂形成离子对络合物。这使得能够进行普通的紫外检测。碘化N - 甲基吡啶在制备简便性和响应方面都被证明是一种合适的试剂。洗脱液由乙腈和水的混合物组成，吡啶盐浓度为0.1 - 0.25 mM。该方法灵敏，具有良好的信噪比，并且在很宽的浓度范围内呈线性响应。所有分析的表面活性剂都被分离，包括一些表面活性剂的非对映体形式。