Zahrobsky Marek, Camporese Davide, Rist Øystein, Carlsen Per H J
Department of Chemistry, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Molecules. 2005 Sep 30;10(9):1179-89. doi: 10.3390/10091179.
A method was developed for the analysis of a number of surfactants which contained no UV-chromophores, using RP-HPLC with Indirect Photometric Detection, IPD. Pyridinium salts such as N-methylpyridinium iodide, N-methyl-2,2'-dipyridinium iodide and N,N'-dimethyl-2,2'-dipyridinium diiodide, were used as the visualization reagents, forming ion-pair complexes with the sulfonate surfactants. This allowed ordinary UV-detection. N-methylpyridinium iodide proved to be a suitable reagent, both with respect to ease of preparation and response. The eluents consisted of mixtures of acetonitrile and water, being 0.1 - 0.25 mM with respect to pyridinium salt. The method was sensitive and exhibited good signal to noise ratios, as well as linear responses over a wide concentration range. All of the analyzed surfactants were separated, including the diastereomeric forms of some of the surfactants.
开发了一种分析多种不含紫外发色团表面活性剂的方法，该方法采用带间接光度检测（IPD）的反相高效液相色谱法。使用吡啶盐，如碘化N - 甲基吡啶、碘化N - 甲基 - 2,2'-联吡啶和二碘化N,N'-二甲基 - 2,2'-联吡啶，作为可视化试剂，与磺酸盐表面活性剂形成离子对络合物。这使得能够进行普通的紫外检测。碘化N - 甲基吡啶在制备简便性和响应方面都被证明是一种合适的试剂。洗脱液由乙腈和水的混合物组成，吡啶盐浓度为0.1 - 0.25 mM。该方法灵敏，具有良好的信噪比，并且在很宽的浓度范围内呈线性响应。所有分析的表面活性剂都被分离，包括一些表面活性剂的非对映体形式。
